Protein Info for b3357 in Escherichia coli BW25113

Name: crp
Annotation: DNA-binding transcriptional dual regulator (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 210 PF00027: cNMP_binding" amino acids 22 to 108 (87 residues), 86.7 bits, see alignment E=1.3e-28 PF13545: HTH_Crp_2" amino acids 142 to 206 (65 residues), 50.4 bits, see alignment E=2.7e-17 PF00325: Crp" amino acids 166 to 197 (32 residues), 37.5 bits, see alignment 2.3e-13

Best Hits

Swiss-Prot: 100% identical to CRP_SHIFL: cAMP-activated global transcriptional regulator CRP (crp) from Shigella flexneri

KEGG orthology group: K10914, CRP/FNR family transcriptional regulator, cyclic AMP receptor protein (inferred from 100% identity to eco:b3357)

Predicted SEED Role

"Cyclic AMP receptor protein" in subsystem CytR regulation or cAMP signaling in bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P0ACJ8 at UniProt or InterPro

Protein Sequence (210 amino acids)

>b3357 DNA-binding transcriptional dual regulator (NCBI) (Escherichia coli BW25113)
MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIVKGSVAVLIKDEEGKEM
ILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKKFRQLIQVNPDILMRLSAQ
MARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTHPDGMQIKITRQEIGQIVGCS
RETVGRILKMLEDQNLISAHGKTIVVYGTR