Protein Info for b3327 in Escherichia coli BW25113

Name: gspF
Annotation: general secretory pathway component, cryptic (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 398 transmembrane" amino acids 164 to 186 (23 residues), see Phobius details amino acids 218 to 236 (19 residues), see Phobius details amino acids 248 to 268 (21 residues), see Phobius details amino acids 368 to 391 (24 residues), see Phobius details PF28597: T2SSF_N" amino acids 2 to 42 (41 residues), 47.8 bits, see alignment 9.8e-17 TIGR02120: type II secretion system protein F" amino acids 3 to 397 (395 residues), 548.5 bits, see alignment E=5.7e-169 PF00482: T2SSF" amino acids 64 to 187 (124 residues), 112.9 bits, see alignment E=1e-36 amino acids 267 to 388 (122 residues), 89.5 bits, see alignment E=1.8e-29

Best Hits

Swiss-Prot: 100% identical to GSPF_ECOLI: Putative type II secretion system protein F (gspF) from Escherichia coli (strain K12)

KEGG orthology group: K02455, general secretion pathway protein F (inferred from 100% identity to eco:b3327)

MetaCyc: 100% identical to type II secretion system protein GspF (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"General secretion pathway protein F"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P41441 at UniProt or InterPro

Protein Sequence (398 amino acids)

>b3327 general secretory pathway component, cryptic (NCBI) (Escherichia coli BW25113)
MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQKSSGVKTRRPRISHSE
LTLFTRQLATLSAAALPLEESLAVIGQQSSNKRLGDVLNQVRSAILEGHPLSDALQHFPT
LFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKLIQSLIYPCMLTTVAIGVVII
LLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQRTGPTLLATVFIVAVGFWLWLKRGN
NRHRFHAMLLRVALIGPLICAINSARYLRTLSILQSSGVPLLDGMNLSTESLNNLEIRQR
LANAAENVRQGNSIHLSLEQTAIFPPMMLYMVASGEKSGQLGTLMVRAADNQETLQQNRI
ALTLSIFEPALIITMALIVLFIVVSVLQPLLQLNSMIN