Protein Info for b3164 in Escherichia coli BW25113

Name: pnp
Annotation: polynucleotide phosphorylase/polyadenylase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 711 TIGR03591: polyribonucleotide nucleotidyltransferase" amino acids 10 to 691 (682 residues), 1092.8 bits, see alignment E=0 PF01138: RNase_PH" amino acids 15 to 144 (130 residues), 100.3 bits, see alignment E=3.6e-32 amino acids 324 to 456 (133 residues), 95.3 bits, see alignment E=1.2e-30 PF03725: RNase_PH_C" amino acids 147 to 210 (64 residues), 59.5 bits, see alignment E=7.7e-20 amino acids 460 to 527 (68 residues), 25.2 bits, see alignment E=4e-09 PF03726: PNPase" amino acids 242 to 320 (79 residues), 95.3 bits, see alignment E=7.7e-31 PF00013: KH_1" amino acids 556 to 614 (59 residues), 40.8 bits, see alignment 4.6e-14 PF00575: S1" amino acids 619 to 690 (72 residues), 84.5 bits, see alignment E=1.7e-27

Best Hits

Swiss-Prot: 100% identical to PNP_ECOHS: Polyribonucleotide nucleotidyltransferase (pnp) from Escherichia coli O9:H4 (strain HS)

KEGG orthology group: K00962, polyribonucleotide nucleotidyltransferase [EC: 2.7.7.8] (inferred from 100% identity to ecr:ECIAI1_3314)

MetaCyc: 100% identical to polynucleotide phosphorylase (Escherichia coli K-12 substr. MG1655)
Exoribonuclease II. [EC: 3.1.13.1]; Polyribonucleotide nucleotidyltransferase. [EC: 3.1.13.1, 2.7.7.8]

Predicted SEED Role

"Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8)" in subsystem Bacterial RNA-metabolizing Zn-dependent hydrolases or Polyadenylation bacterial (EC 2.7.7.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.13.1

Use Curated BLAST to search for 2.7.7.8 or 3.1.13.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P05055 at UniProt or InterPro

Protein Sequence (711 amino acids)

>b3164 polynucleotide phosphorylase/polyadenylase (RefSeq) (Escherichia coli BW25113)
MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTVVGQKKAKPGQDFFPLT
VNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPLFPEGFVNEVQVIATVVSV
NPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQYVLNPTQDELKESKLDLVVA
GTEAAVLMVESEAQLLSEDQMLGAVVFGHEQQQVVIQNINELVKEAGKPRWDWQPEPVNE
ALNARVAALAEARLSDAYRITDKQERYAQVDVIKSETIATLLAEDETLDENELGEILHAI
EKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVLPRTHGSALFTRGETQALVTATLGTAR
DAQVLDELMGERTDTFLFHYNFPPYSVGETGMVGSPKRREIGHGRLAKRGVLAVMPDMDK
FPYTVRVVSEITESNGSSSMASVCGASLALMDAGVPIKAAVAGIAMGLVKEGDNYVVLSD
ILGDEDHLGDMDFKVAGSRDGISALQMDIKIEGITKEIMQVALNQAKGARLHILGVMEQA
INAPRGDISEFAPRIHTIKINPDKIKDVIGKGGSVIRALTEETGTTIEIEDDGTVKIAAT
DGEKAKHAIRRIEEITAEIEVGRVYTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVE
KVTDYLQMGQEVPVKVLEVDRQGRIRLSIKEATEQSQPAAAPEAPAAEQGE