Protein Info for b3077 in Escherichia coli BW25113

Name: ebgC
Annotation: cryptic beta-D-galactosidase, beta subunit (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 149 PF04074: DUF386" amino acids 3 to 140 (138 residues), 89.4 bits, see alignment E=1.2e-29

Best Hits

Swiss-Prot: 100% identical to EBGC_ECOLI: Evolved beta-galactosidase subunit beta (ebgC) from Escherichia coli (strain K12)

KEGG orthology group: K12112, evolved beta-galactosidase subunit beta (inferred from 100% identity to eco:b3077)

Predicted SEED Role

"Evolved beta-D-galactosidase, beta subunit" in subsystem Lactose utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P0AC73 at UniProt or InterPro

Protein Sequence (149 amino acids)

>b3077 cryptic beta-D-galactosidase, beta subunit (NCBI) (Escherichia coli BW25113)
MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDSLTYRVETDSATDALFT
GHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLKGCGETVEVHEGQIVICDI
HEAYRFICNNAVKKVVLKVTIEDGYFHNK