Protein Info for b2799 in Escherichia coli BW25113

Name: fucO
Annotation: L-1,2-propanediol oxidoreductase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02638: lactaldehyde reductase
  • amino acids 2 to 378 (377 residues), 663.9 bits, E=3.1e-204
• PF00465: Fe-ADH
  • amino acids 11 to 374 (364 residues), 393.9 bits, E=6.5e-122
• PF13685: Fe-ADH_2
  • amino acids 13 to 111 (99 residues), 40.3 bits, E=3.2e-14

Best Hits

Swiss-Prot: 100% identical to FUCO_ECO57: Lactaldehyde reductase (fucO) from Escherichia coli O157:H7

KEGG orthology group: K00048, lactaldehyde reductase [EC: 1.1.1.77] (inferred from 100% identity to eco:b2799)

MetaCyc: 100% identical to L-1,2-propanediol oxidoreductase (Escherichia coli K-12 substr. MG1655)
Lactaldehyde reductase. [EC: 1.1.1.77]; 1.1.1.77 [EC: 1.1.1.77]

Predicted SEED Role

"Lactaldehyde reductase (EC 1.1.1.77)" in subsystem L-fucose utilization or L-rhamnose utilization (EC 1.1.1.77)

MetaCyc Pathways

• superpathway of fucose and rhamnose degradation (12/12 steps found)
• superpathway of glycol metabolism and degradation (7/7 steps found)
• ethylene glycol degradation (2/2 steps found)
• L-lactaldehyde degradation (anaerobic) (1/1 steps found)
• nucleoside and nucleotide degradation (halobacteria) (3/6 steps found)
• methylglyoxal degradation VI (1/4 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.77

Sequence Analysis Tools

See P0A9S1 at UniProt or InterPro

Protein Sequence (382 amino acids)

>b2799 L-1,2-propanediol oxidoreductase (NCBI) (Escherichia coli BW25113)
MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQCGVVAKVTDKMDAAGLA
WAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDTCKAIGIISNNPEFADVRS
LEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRRKFVCVDPHDIPQVAFIDADM
MDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHIKAIEIIAGALRGSVAGDKDAGE
EMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPHGVANAILLPHVMRYNADFTGEKYR
DIARVMGVKVEGMSLEEARNAAVEAVFALNRDVGIPPHLRDVGVRKEDIPALAQAALDDV
CTGGNPREATLEDIVELYHTAW