Protein Info for b2739 in Escherichia coli BW25113

Name: ygbM
Annotation: hypothetical protein (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01261: AP_endonuc_2
  • amino acids 21 to 256 (236 residues), 172.6 bits, E=6.3e-55

Best Hits

Swiss-Prot: 100% identical to OTNI_ECOLI: 2-oxo-tetronate isomerase (otnI) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to eco:b2739)

MetaCyc: 60% identical to 2-dehydrotetronate isomerase (Haemophilus influenzae Rd KW20)
RXN-18592 [EC: 5.3.1.35]; 5.3.1.35 [EC: 5.3.1.35]

Predicted SEED Role

"Hydroxypyruvate isomerase (EC 5.3.1.22)" (EC 5.3.1.22)

MetaCyc Pathways

• L-threonate degradation (3/4 steps found)
• D-erythronate degradation II (2/4 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.3.1.22

Use Curated BLAST to search for 5.3.1.22 or 5.3.1.35

Sequence Analysis Tools

See Q46891 at UniProt or InterPro

Protein Sequence (258 amino acids)

>b2739 hypothetical protein (NCBI) (Escherichia coli BW25113)
MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYNYSTLQIQKQLEQNHLTLALFN
TAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQVHVMAGVVPAGEDAERYRA
VFIDNIRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQALAIVEEVARDNVFIQLDTF
HAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGEINYPWLFRLFDEVGYQGWIGCE
YKPRGLTEEGLGWFDAWR