Protein Info for b2697 in Escherichia coli BW25113

Name: alaS
Annotation: alanyl-tRNA synthetase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01411: tRNA-synt_2c
  • amino acids 7 to 555 (549 residues), 804 bits, E=1.1e-245
• TIGR00344: alanine--tRNA ligase
  • amino acids 7 to 854 (848 residues), 1205.1 bits, E=0
• PF07973: tRNA_SAD
  • amino acids 653 to 696 (44 residues), 62 bits, 6.4e-21
• PF02272: DHHA1
  • amino acids 732 to 871 (140 residues), 116.4 bits, E=2.1e-37

Best Hits

Swiss-Prot: 100% identical to SYA_ECOLI: Alanine--tRNA ligase (alaS) from Escherichia coli (strain K12)

KEGG orthology group: K01872, alanyl-tRNA synthetase [EC: 6.1.1.7] (inferred from 100% identity to ebd:ECBD_1028)

MetaCyc: 100% identical to alanine--tRNA ligase/DNA-binding transcriptional repressor (Escherichia coli K-12 substr. MG1655)
Alanine--tRNA ligase. [EC: 6.1.1.7]; 6.1.1.7 [EC: 6.1.1.7]; 6.1.1.7 [EC: 6.1.1.7]; RXN-19726 [EC: 6.1.1.7, 3.1.1.96]; 3.1.1.- [EC: 6.1.1.7, 3.1.1.96]

Predicted SEED Role

"Alanyl-tRNA synthetase (EC 6.1.1.7)" (EC 6.1.1.7)

MetaCyc Pathways

• superpathway of chorismate metabolism (54/59 steps found)
• tRNA charging (21/21 steps found)
• enterobactin biosynthesis (7/11 steps found)
• bacillibactin biosynthesis (6/12 steps found)
• shinorine biosynthesis (3/8 steps found)
• vanchrobactin biosynthesis (3/8 steps found)
• ergotamine biosynthesis (4/12 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• superpathway of ergotamine biosynthesis (5/18 steps found)
• sulfazecin biosynthesis (1/16 steps found)
• phosalacine biosynthesis (2/25 steps found)
• phosphinothricin tripeptide biosynthesis (2/25 steps found)
• corallopyronin A biosynthesis (2/30 steps found)
• colibactin biosynthesis (7/38 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Aminoacyl-tRNA biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.1.96 or 6.1.1.7

Sequence Analysis Tools

See P00957 at UniProt or InterPro

Protein Sequence (876 amino acids)

>b2697 alanyl-tRNA synthetase (NCBI) (Escherichia coli BW25113)
MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYS
RATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTSEKW
FALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDNKGAPYASDNFWQMGDTGPCG
PCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMG
LERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDLSNKSLRVIADHIRSCAFLIADGVM
PSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKLVGPLIDVMGSAGEDLKRQQAQVEQVL
KTEEEQFARTLERGLALLDEELAKLSGDTLDGETAFRLYDTYGFPVDLTADVCRERNIKV
DEAGFEAAMEEQRRRAREASGFGADYNAMIRVDSASEFKGYDHLELNGKVTALFVDGKAV
DAINAGQEAVVVLDQTPFYAESGGQVGDKGELKGANFSFAVEDTQKYGQAIGHIGKLAAG
SLKVGDAVQADVDEARRARIRLNHSATHLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSH
NEAMKPEEIRAVEDLVNTQIRRNLPIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGD
FSTELCGGTHASRTGDIGLFRIISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHL
LKGDSNNLADKVRSVLERTRQLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSG
VEPKMLRTMVDDLKNQLGSTIIVLATVVEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVG
GKGGGRPDMAQAGGTDAAALPAALASVKGWVSAKLQ