Protein Info for b2615 in Escherichia coli BW25113
Name: yfjB
Annotation: inorganic polyphosphate/ATP-NAD kinase (NCBI)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to NADK_ECO55: NAD kinase (nadK) from Escherichia coli (strain 55989 / EAEC)
KEGG orthology group: K00858, NAD+ kinase [EC: 2.7.1.23] (inferred from 100% identity to eco:b2615)MetaCyc: 100% identical to NAD kinase (Escherichia coli K-12 substr. MG1655)
2.7.1.M29,2.7.1.23 [EC: 2.7.1.23, 2.7.1.M29]
Predicted SEED Role
"NAD kinase (EC 2.7.1.23)" in subsystem NAD and NADP cofactor biosynthesis global (EC 2.7.1.23)
MetaCyc Pathways
- NAD phosphorylation and dephosphorylation (3/3 steps found)
- NAD phosphorylation and transhydrogenation (2/2 steps found)
- cytosolic NADPH production (yeast) (4/5 steps found)
- NADP biosynthesis (1/1 steps found)
- superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) (6/8 steps found)
- NAD(P)/NADPH interconversion (4/6 steps found)
- mitochondrial NADPH production (yeast) (3/5 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 2.7.1.23 or 2.7.1.M29
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See P0A7B3 at UniProt or InterPro
Protein Sequence (292 amino acids)
>b2615 inorganic polyphosphate/ATP-NAD kinase (NCBI) (Escherichia coli BW25113) MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIAHELQLKNVKTGTLAEI GQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLTDLDPDNAQQQLADVLEGH YISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMIEFEVYIDEIFAFSQRSDGLI ISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSARPLVINSSSTIRLRFSHRRNDL EISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYSYFNTLSTKLGWSKKLF