Protein Info for b2599 in Escherichia coli BW25113
Name: pheA
Annotation: fused chorismate mutase P/prephenate dehydratase (NCBI)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to CMPDT_SHIFL: Bifunctional chorismate mutase/prephenate dehydratase (pheA) from Shigella flexneri
KEGG orthology group: K14170, chorismate mutase / prephenate dehydratase [EC: 4.2.1.51 5.4.99.5] (inferred from 100% identity to eco:b2599)MetaCyc: 100% identical to fused chorismate mutase/prephenate dehydratase (Escherichia coli K-12 substr. MG1655)
Chorismate mutase. [EC: 5.4.99.5]; Prephenate dehydratase. [EC: 5.4.99.5, 4.2.1.51]
Predicted SEED Role
"Chorismate mutase I (EC 5.4.99.5) / Prephenate dehydratase (EC 4.2.1.51)" in subsystem Chorismate Synthesis or Phenylalanine and Tyrosine Branches from Chorismate (EC 4.2.1.51, EC 5.4.99.5)
MetaCyc Pathways
- superpathway of chorismate metabolism (54/59 steps found)
- superpathway of aromatic amino acid biosynthesis (18/18 steps found)
- superpathway of L-phenylalanine biosynthesis (10/10 steps found)
- superpathway of L-tyrosine biosynthesis (10/10 steps found)
- L-phenylalanine biosynthesis I (3/3 steps found)
- L-tyrosine biosynthesis I (3/3 steps found)
- L-phenylalanine biosynthesis III (cytosolic, plants) (2/2 steps found)
- L-tyrosine biosynthesis III (3/4 steps found)
- L-phenylalanine biosynthesis II (2/4 steps found)
- L-tyrosine biosynthesis II (2/4 steps found)
- superpathway of L-phenylalanine and L-tyrosine biosynthesis (2/5 steps found)
- bacilysin biosynthesis (1/10 steps found)
- salinosporamide A biosynthesis (3/15 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of phenylpropanoids
- Phenylalanine, tyrosine and tryptophan biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 5.4.99.5
Use Curated BLAST to search for 4.2.1.51 or 5.4.99.5
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See P0A9J8 at UniProt or InterPro
Protein Sequence (386 amino acids)
>b2599 fused chorismate mutase P/prephenate dehydratase (NCBI) (Escherichia coli BW25113) MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHRPVRDIDRERDLLERLI TLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPHSARIAFLGPKGSYSHLAA RQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIENTSSGAINDVYDLLQHTSLSI VGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSKFLNRYPHWKIEYTESTSAAMEK VAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQNFTRFVVLARKAINVSDQVPAKTTL LMATGQQAGALVEALLVLRNHNLIMTRLESRPIHGNPWEEMFYLDIQANLESAEMQKALK ELGEITRSMKVLGCYPSENVVPVDPT