Protein Info for b2539 in Escherichia coli BW25113

Name: hcaF
Annotation: 3-phenylpropionate dioxygenase, small (beta) subunit (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to HCAF_ECO55: 3-phenylpropionate/cinnamic acid dioxygenase subunit beta (hcaF) from Escherichia coli (strain 55989 / EAEC)

KEGG orthology group: K05709, small terminal subunit of phenylpropionate dioxygenase [EC: 1.14.12.19] (inferred from 100% identity to eco:b2539)

MetaCyc: 100% identical to putative 3-phenylpropionate/cinnamate dioxygenase subunit beta (Escherichia coli K-12 substr. MG1655)
3-phenylpropanoate dioxygenase. [EC: 1.14.12.19]; 1.14.12.19 [EC: 1.14.12.19]

Predicted SEED Role

"3-phenylpropionate dioxygenase, beta subunit (EC 1.14.12.19)" in subsystem Cinnamic Acid Degradation or Flavohaemoglobin or Phenylpropionate Degradation (EC 1.14.12.19)

MetaCyc Pathways

3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation (8/8 steps found)
3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation to 2-hydroxypentadienoate (5/5 steps found)
cinnamate and 3-hydroxycinnamate degradation to 2-hydroxypentadienoate (5/5 steps found)

KEGG Metabolic Maps

Phenylalanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.14.12.19

Use Curated BLAST to search for 1.14.12.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q47140 at UniProt or InterPro

Protein Sequence (172 amino acids)

>b2539 3-phenylpropionate dioxygenase, small (beta) subunit (NCBI) (Escherichia coli BW25113)
MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTMRTTVNAQTRDRRKGVQ
PPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNCQISETDIPNVFAVRVNYL
LYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQAVITSHNLSVLF