Protein Info for b2523 in Escherichia coli BW25113

Name: pepB
Annotation: putative peptidase (VIMSS)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF12404: DUF3663
  • amino acids 5 to 81 (77 residues), 113.2 bits, E=4.6e-37
• PF00883: Peptidase_M17
  • amino acids 109 to 421 (313 residues), 348.3 bits, E=4.1e-108

Best Hits

Swiss-Prot: 100% identical to PEPB_ECOLI: Peptidase B (pepB) from Escherichia coli (strain K12)

KEGG orthology group: K07751, PepB aminopeptidase [EC: 3.4.11.23] (inferred from 100% identity to eco:b2523)

MetaCyc: 100% identical to aminopeptidase B (Escherichia coli K-12 substr. MG1655)
Cytosol nonspecific dipeptidase. [EC: 3.4.13.18]; PepB aminopeptidase. [EC: 3.4.13.18, 3.4.11.23]

Predicted SEED Role

"Peptidase B (EC 3.4.11.23)" (EC 3.4.11.23)

MetaCyc Pathways

• muropeptide degradation (4/4 steps found)
• γ-glutamyl cycle (5/6 steps found)
• glutathione degradation (DUG pathway) (2/2 steps found)

KEGG Metabolic Maps

• Glutathione metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.11.23 or 3.4.13.18

Sequence Analysis Tools

See P37095 at UniProt or InterPro

Protein Sequence (427 amino acids)

>b2523 putative peptidase (VIMSS) (Escherichia coli BW25113)
MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLIQRAARKIDGLGIKHVQ
LSGEGWDADRCWAFWQGYKAPKGTRKVVWPDLDDAQRQELDNRLMIIDWVRDTINAPAEE
LGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGLHTVGRGSERSPVLLALDYNP
TGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSDMGGAATVTGALAFAITRGLNKR
VKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNTDAEGRLVLADGLIDASAQKPEMII
DAATLTGAAKTALGNDYHALFSFDDALAGRLLASAAQENEPFWRLPLAEFHRSQLPSNFA
ELNNTGSAAYPAGASTAAGFLSHFVENYQQGWLHIDCSATYRKAPVEQWSAGATGLGVRT
IANLLTA