Protein Info for b2290 in Escherichia coli BW25113
Name: yfbQ
Annotation: aspartate aminotransferase (NCBI)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to ALAA_ECOL6: Glutamate-pyruvate aminotransferase AlaA (alaA) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
KEGG orthology group: K14260, alanine-synthesizing transaminase [EC: 2.6.1.2 2.6.1.66] (inferred from 100% identity to eco:b2290)MetaCyc: 100% identical to glutamate--pyruvate aminotransferase AlaA (Escherichia coli K-12 substr. MG1655)
Alanine transaminase. [EC: 2.6.1.2]
Predicted SEED Role
"Alanine transaminase (EC 2.6.1.2)" (EC 2.6.1.2)
MetaCyc Pathways
- superpathway of L-alanine biosynthesis (4/4 steps found)
- L-alanine biosynthesis I (2/2 steps found)
- L-alanine degradation V (oxidative Stickland reaction) (2/2 steps found)
- L-alanine biosynthesis II (1/1 steps found)
- L-alanine degradation III (1/1 steps found)
- C4 photosynthetic carbon assimilation cycle, NAD-ME type (8/11 steps found)
- anaerobic energy metabolism (invertebrates, cytosol) (5/7 steps found)
- L-alanine degradation II (to D-lactate) (2/3 steps found)
- pyruvate fermentation to acetate and alanine (2/3 steps found)
- superpathway of anaerobic energy metabolism (invertebrates) (12/17 steps found)
- C4 photosynthetic carbon assimilation cycle, PEPCK type (9/14 steps found)
- superpathway of L-alanine fermentation (Stickland reaction) (5/9 steps found)
- L-alanine degradation VI (reductive Stickland reaction) (2/6 steps found)
- Methanobacterium thermoautotrophicum biosynthetic metabolism (22/56 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Carbon fixation in photosynthetic organisms
- Glutamate metabolism
- Valine, leucine and isoleucine biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 2.6.1.66
Use Curated BLAST to search for 2.6.1.2 or 2.6.1.66
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See P0A959 at UniProt or InterPro
Protein Sequence (405 amino acids)
>b2290 aspartate aminotransferase (NCBI) (Escherichia coli BW25113) MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPAPFGFDAPDEILVDVIR NLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGNGVSELIVQAMQALLNSGD EMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDDIRAKITPRTRGIVIINPNNP TGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDAEHHSIAPLAPDLLTITFNGLSK TYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRLCANVPAQHAIQTALGGYQSISEFI TPGGRLYEQRNRAWELINDIPGVSCVKPRGALYMFPKIDAKRFNIHDDQKMVLDFLLQEK VLLVQGTAFNWPWPDHFRIVTLPRVDDIELSLSKFARFLSGYHQL