Protein Info for b2260 in Escherichia coli BW25113

Name: menE
Annotation: O-succinylbenzoic acid--CoA ligase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to MENE_ECOLI: 2-succinylbenzoate--CoA ligase (menE) from Escherichia coli (strain K12)

KEGG orthology group: K01911, O-succinylbenzoic acid--CoA ligase [EC: 6.2.1.26] (inferred from 100% identity to eco:b2260)

MetaCyc: 100% identical to o-succinylbenzoate--CoA ligase (Escherichia coli K-12 substr. MG1655)
o-succinylbenzoate--CoA ligase. [EC: 6.2.1.26]

Predicted SEED Role

"O-succinylbenzoic acid--CoA ligase (EC 6.2.1.26)" in subsystem Menaquinone and Phylloquinone Biosynthesis (EC 6.2.1.26)

MetaCyc Pathways

superpathway of chorismate metabolism (54/59 steps found)
superpathway of menaquinol-8 biosynthesis I (10/10 steps found)
superpathway of demethylmenaquinol-8 biosynthesis I (9/9 steps found)
2-carboxy-1,4-naphthoquinol biosynthesis (7/7 steps found)
superpathway of menaquinol-10 biosynthesis (9/10 steps found)
superpathway of menaquinol-11 biosynthesis (9/10 steps found)
superpathway of menaquinol-12 biosynthesis (9/10 steps found)
superpathway of menaquinol-13 biosynthesis (9/10 steps found)
superpathway of menaquinol-6 biosynthesis (9/10 steps found)
superpathway of menaquinol-7 biosynthesis (9/10 steps found)
superpathway of menaquinol-9 biosynthesis (9/10 steps found)
superpathway of demethylmenaquinol-6 biosynthesis I (8/9 steps found)
superpathway of demethylmenaquinol-9 biosynthesis (8/9 steps found)
superpathway of phylloquinol biosynthesis (7/15 steps found)

KEGG Metabolic Maps

Ubiquinone and menaquinone biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.2.1.26

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P37353 at UniProt or InterPro

Protein Sequence (451 amino acids)

>b2260 O-succinylbenzoic acid--CoA ligase (NCBI) (Escherichia coli BW25113)
MIFSDWPWRHWRQVRGETIALRLNDEQLNWRELCARVDELASGFAVQGVVEGSGVMLRAW
NTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQFALVPDGENTFPALTSLHI
QLVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHLASAQGVLSLIPFGDHDDWLL
SLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSL
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKI
VNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYATRDRGEMHNGKLTIVGRLDNLFFSGGE
GIQPEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVMEYDHESVDLSEWVKDKLARFQQ
PVRWLTLPPELKNGGIKISRQALKEWVQRQQ