Protein Info for b2247 in Escherichia coli BW25113

Name: yfaW
Annotation: predicted enolase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 401 PF02746: MR_MLE_N" amino acids 67 to 157 (91 residues), 33.3 bits, see alignment E=5.2e-12 PF13378: MR_MLE_C" amino acids 179 to 392 (214 residues), 128.6 bits, see alignment E=3e-41

Best Hits

Swiss-Prot: 100% identical to RHMD_ECODH: L-rhamnonate dehydratase (rhmD) from Escherichia coli (strain K12 / DH10B)

KEGG orthology group: K12661, L-rhamnonate dehydratase [EC: 4.2.1.90] (inferred from 100% identity to eco:b2247)

MetaCyc: 100% identical to L-rhamnonate dehydratase (Escherichia coli K-12 substr. MG1655)
L-rhamnonate dehydratase. [EC: 4.2.1.90]

Predicted SEED Role

"L-rhamnonate dehydratase (EC 4.2.1.90)" in subsystem L-rhamnose utilization (EC 4.2.1.90)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.90

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P77215 at UniProt or InterPro

Protein Sequence (401 amino acids)

>b2247 predicted enolase (RefSeq) (Escherichia coli BW25113)
MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATPMSKYRDYEQSRQSFGI
NVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEGKCVSDIKLIHDQMLSATL
YYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAVRDEIQFYATGARPDLAKEMG
FIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFWLMLDCWMSQDVNYATKLAHACA
PYNLKWIEECLPPQQYESYRELKRNAPVGMMVTSGEHHGTLQSFRTLSETGIDIMQPDVG
WCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHAVITFTNTPFSEFLMTSPDCSTMRPQF
DPILLNEPVPVNGRIHKSVLDKPGFGVELNRDCNLKRPYSH