Protein Info for b2132 in Escherichia coli BW25113

Name: bglX
Annotation: beta-D-glucoside glucohydrolase, periplasmic (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 765 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF00933: Glyco_hydro_3" amino acids 46 to 352 (307 residues), 367.2 bits, see alignment E=1.3e-113 PF01915: Glyco_hydro_3_C" amino acids 395 to 648 (254 residues), 210.1 bits, see alignment E=5.2e-66 PF14310: Fn3-like" amino acids 686 to 754 (69 residues), 88.1 bits, see alignment E=5.1e-29

Best Hits

Swiss-Prot: 100% identical to BGLX_ECOLI: Periplasmic beta-glucosidase (bglX) from Escherichia coli (strain K12)

KEGG orthology group: K05349, beta-glucosidase [EC: 3.2.1.21] (inferred from 100% identity to ecv:APECO1_4417)

MetaCyc: 100% identical to beta-D-glucoside glucohydrolase, periplasmic (Escherichia coli K-12 substr. MG1655)
Beta-glucosidase. [EC: 3.2.1.21]

Predicted SEED Role

"Periplasmic beta-glucosidase (EC 3.2.1.21)" in subsystem Beta-Glucoside Metabolism (EC 3.2.1.21)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P33363 at UniProt or InterPro

Protein Sequence (765 amino acids)

>b2132 beta-D-glucoside glucohydrolase, periplasmic (NCBI) (Escherichia coli BW25113)
MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKKMTVDEKIGQLRLISVG
PDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRLKIPLFFAYDVLHGQRTVF
PISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVDVSRDPRWGRASEGFGEDTYL
TSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEGGKEYNTVDMSPQRLFNDYMPPY
KAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRDQWGFKGITVSDHGAIKELIKHGTA
ADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKSGKVTMAELDDAARHVLNVKYDMGLFN
DPYSHLGPKESDPVDTNAESRLHRKEAREVARESLVLLKNRLETLPLKKSATIAVVGPLA
DSKRDVMGSWSAAGVADQSVTVLTGIKNAVGENGKVLYAKGANVTSDKGIIDFLNQYEEA
VKVDPRSPQEMIDEAVQTAKQSDVVVAVVGEAQGMAHEASSRTDITIPQSQRDLIAALKA
TGKPLVLVLMNGRPLALVKEDQQADAILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFP
RSVGQIPVYYSHLNTGRPYNADKPNKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSA
PTMKRDGKVTASVQVTNTGKREGATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQT
VSFPIDIEALKFWNQQMKYDAEPGKFNVFIGTDSARVKKGEFELL