Protein Info for b2037 in Escherichia coli BW25113

Name: rfbX
Annotation: predicted polisoprenol-linked O-antigen transporter (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 415 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 42 to 63 (22 residues), see Phobius details amino acids 84 to 106 (23 residues), see Phobius details amino acids 117 to 138 (22 residues), see Phobius details amino acids 146 to 166 (21 residues), see Phobius details amino acids 172 to 196 (25 residues), see Phobius details amino acids 217 to 242 (26 residues), see Phobius details amino acids 263 to 282 (20 residues), see Phobius details amino acids 294 to 316 (23 residues), see Phobius details amino acids 327 to 349 (23 residues), see Phobius details amino acids 361 to 380 (20 residues), see Phobius details amino acids 386 to 407 (22 residues), see Phobius details PF01943: Polysacc_synt" amino acids 9 to 278 (270 residues), 271.7 bits, see alignment E=1.1e-84 PF13440: Polysacc_synt_3" amino acids 33 to 328 (296 residues), 32.4 bits, see alignment E=9e-12 PF14667: Polysacc_synt_C" amino acids 330 to 409 (80 residues), 29.3 bits, see alignment E=1.3e-10

Best Hits

Swiss-Prot: 100% identical to RFBX_ECOLI: Putative O-antigen transporter (rfbX) from Escherichia coli (strain K12)

KEGG orthology group: K03328, polysaccharide transporter, PST family (inferred from 100% identity to eco:b2037)

MetaCyc: 100% identical to polyisoprenol-linked O16-antigen repeat unit flippase (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Membrane protein involved in the export of O-antigen, teichoic acid lipoteichoic acids"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P37746 at UniProt or InterPro

Protein Sequence (415 amino acids)

>b2037 predicted polisoprenol-linked O-antigen transporter (NCBI) (Escherichia coli BW25113)
MNTNKLSLRRNVIYLAVVQGSNYLLPLLTFPYLVRTLGPENFGIFGFCQATMLYMIMFVE
YGFNLTATQSIAKAADSKDKVTSIFWAVIFSKIVLIVITLIFLTSMTLLVPEYNKHAVII
WSFVPALVGNLIYPIWLFQGKEKMKWLTLSSILSRLAIIPLTFIFVNTKSDIAIAGFIQS
SANLVAGIIALAIVVHEGWIGKVTLSLHNVRRSLADGFHVFISTSAISLYSTGIVIILGF
ISGPTSVGNFNAANTIRNALQGLLNPITQAIYPRISSTLVLNRVKGVILIKKSLTCLSLI
GGAFSLILLLGASILVKISIGPGYDNAVIVLMIISPLPFLISLSNVYGIQVMLTHNYKKE
FSKILIAAGLLSLLLIFPLTTLFKEIGAAITLLATECLVTSLMLMFVRNNKLLVC