Protein Info for b1876 in Escherichia coli BW25113

Name: argS
Annotation: arginyl-tRNA synthetase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 577 TIGR00456: arginine--tRNA ligase" amino acids 3 to 577 (575 residues), 728.1 bits, see alignment E=3.2e-223 PF03485: Arg_tRNA_synt_N" amino acids 7 to 87 (81 residues), 78.7 bits, see alignment E=6.4e-26 PF00750: tRNA-synt_1d" amino acids 95 to 446 (352 residues), 601.2 bits, see alignment E=8.2e-185 PF05746: DALR_1" amino acids 460 to 577 (118 residues), 127.1 bits, see alignment E=5.6e-41

Best Hits

Swiss-Prot: 100% identical to SYR_ECOBW: Arginine--tRNA ligase (argS) from Escherichia coli (strain K12 / MC4100 / BW2952)

KEGG orthology group: K01887, arginyl-tRNA synthetase [EC: 6.1.1.19] (inferred from 100% identity to eco:b1876)

MetaCyc: 100% identical to arginine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Arginine--tRNA ligase. [EC: 6.1.1.19]

Predicted SEED Role

"Arginyl-tRNA synthetase (EC 6.1.1.19)" (EC 6.1.1.19)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P11875 at UniProt or InterPro

Protein Sequence (577 amino acids)

>b1876 arginyl-tRNA synthetase (NCBI) (Escherichia coli BW25113)
MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGMMAVAKKLGMAPRQLAE
QVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALASDRLGVATPEKQTIVVDYS
APNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVGDWGTQFGMLIAWLEKQQQEN
AGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQSGDEYFREMWRKLVDITMTQNQ
ITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKGLAVESEGATVVFLDEFKNKEGEPM
GVIIQKKDGGYLYTTTDIACAKYRYETLHADRVLYYIDSRQHQHLMQAWAIVRKAGYVPE
SVPLEHHMFGMMLGKDGKPFKTRAGGTVKLADLLDEALERARRLVAEKNPDMPADELEKL
ANAVGIGAVKYADLSKNRTTDYIFDWDNMLAFEGNTAPYMQYAYTRVLSVFRKAEIDEEQ
LAAAPVIIREDREAQLAARLLQFEETLTVVAREGTPHVMCAYLYDLAGLFSGFYEHCPIL
SAENEEVRNSRLKLAQLTAKTLKLGLDTLGIETVERM