Protein Info for b1871 in Escherichia coli BW25113

Name: yecP
Annotation: predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08003: Methyltransf_9
  • amino acids 7 to 322 (316 residues), 575.2 bits, E=7.4e-177
• TIGR00452: tRNA (mo5U34)-methyltransferase
  • amino acids 8 to 321 (314 residues), 490.1 bits, E=1e-151
• PF13489: Methyltransf_23
  • amino acids 111 to 269 (159 residues), 39.7 bits, E=1.1e-13
• PF13847: Methyltransf_31
  • amino acids 123 to 228 (106 residues), 31.3 bits, E=4.3e-11
• PF13649: Methyltransf_25
  • amino acids 126 to 204 (79 residues), 29.5 bits, E=2.5e-10
• PF08241: Methyltransf_11
  • amino acids 127 to 223 (97 residues), 25.4 bits, E=4.4e-09

Best Hits

Swiss-Prot: 100% identical to CMOB_ECO45: tRNA U34 carboxymethyltransferase (cmoB) from Escherichia coli O45:K1 (strain S88 / ExPEC)

KEGG orthology group: K15257, tRNA (mo5U34)-methyltransferase [EC: 2.1.1.-] (inferred from 100% identity to eco:b1871)

MetaCyc: 100% identical to tRNA U34 carboxymethyltransferase (Escherichia coli K-12 substr. MG1655)
RXN0-7067

Predicted SEED Role

"tRNA (5-methoxyuridine) 34 synthase"

MetaCyc Pathways

• 5-(methoxycarbonylmethoxy)uridine biosynthesis (3/3 steps found)

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Anthocyanin biosynthesis
• Benzoxazinone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway
• Carotenoid biosynthesis - General
• Flavonoid biosynthesis
• Histidine metabolism
• Insect hormone biosynthesis
• Naphthalene and anthracene degradation
• Phenylpropanoid biosynthesis
• Porphyrin and chlorophyll metabolism
• Tryptophan metabolism
• Tyrosine metabolism
• Ubiquinone and menaquinone biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.-

Sequence Analysis Tools

See P76291 at UniProt or InterPro

Protein Sequence (323 amino acids)

>b1871 predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI) (Escherichia coli BW25113)
MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSNAVEFLPEIKPYRLDLL
HSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNIDTEWRSDWKWDRVLPHLSD
LTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQFEAVRKLLGNDQRAHLLPLG
IEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVNEGELVLETLVIDGDENTVLVPG
DRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVSVTTTEEQRRTEWMVTESLADFLDP
HDPGKTVEGYPAPKRAVLIARKP