Protein Info for b1819 in Escherichia coli BW25113

Name: manZ
Annotation: mannose-specific enzyme IID component of PTS (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to PTND_ECO57: PTS system mannose-specific EIID component (manZ) from Escherichia coli O157:H7

KEGG orthology group: K02796, PTS system, mannose-specific IID component (inferred from 100% identity to eco:b1819)

MetaCyc: 100% identical to mannose-specific PTS enzyme IID component (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-157 [EC: 2.7.1.199]; 2.7.1.199 [EC: 2.7.1.199]; TRANS-RXN-167 [EC: 2.7.1.199, 2.7.1.193]; TRANS-RXN-165 [EC: 2.7.1.199, 2.7.1.193, 2.7.1.191]; 2.7.1.191 [EC: 2.7.1.199, 2.7.1.193, 2.7.1.191]

Predicted SEED Role

"PTS system, mannose-specific IID component"

MetaCyc Pathways

superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (25/26 steps found)
superpathway of glycolysis and the Entner-Doudoroff pathway (17/17 steps found)
glycolysis I (from glucose 6-phosphate) (13/13 steps found)
D-mannose degradation I (2/2 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.191 or 2.7.1.193 or 2.7.1.199

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P69805 at UniProt or InterPro

Protein Sequence (283 amino acids)

>b1819 mannose-specific enzyme IID component of PTS (NCBI) (Escherichia coli BW25113)
MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCFSMVPAIRRLYPENNEA
RKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDGAINGIKVGLMGPLAGVGD
PIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLATRYYGVAYGYSKGIDIVKDM
GGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSRITDQTGKEHVTTVQTILDQLMP
GLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGYACGLLGL