Protein Info for b1761 in Escherichia coli BW25113

Name: gdhA
Annotation: glutamate dehydrogenase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02812: ELFV_dehydrog_N
  • amino acids 57 to 184 (128 residues), 168.5 bits, E=5.8e-54
• PF00208: ELFV_dehydrog
  • amino acids 202 to 445 (244 residues), 302.4 bits, E=2.7e-94

Best Hits

Swiss-Prot: 100% identical to DHE4_ECOLI: NADP-specific glutamate dehydrogenase (gdhA) from Escherichia coli (strain K12)

KEGG orthology group: K00262, glutamate dehydrogenase (NADP+) [EC: 1.4.1.4] (inferred from 100% identity to eco:b1761)

MetaCyc: 100% identical to glutamate dehydrogenase (Escherichia coli K-12 substr. MG1655)
Glutamate dehydrogenase (NADP(+)). [EC: 1.4.1.4]

Predicted SEED Role

"NADP-specific glutamate dehydrogenase (EC 1.4.1.4)" in subsystem Arginine and Ornithine Degradation or Glutamate dehydrogenases or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis or Proline Synthesis (EC 1.4.1.4)

MetaCyc Pathways

• L-glutamate biosynthesis III (1/1 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.1.4

Sequence Analysis Tools

See P00370 at UniProt or InterPro

Protein Sequence (447 amino acids)

>b1761 glutamate dehydrogenase (NCBI) (Escherichia coli BW25113)
MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPKYRQMSLLERLVEPERV
IQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVNLSILKFLGFEQTFKNALT
TLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGADTDVPAGDIGVGGREVGFMA
GMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYFTEAMLKRHGMGFEGMRVSVSGS
GNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTKEKLARLIEIKASRDGRVADYAKEF
GLVYLEGQQPWSLPVDIALPCATQNELDVDAAHQLIANGVKAVAEGANMPTTIEATELFQ
QAGVLFAPGKAANAGGVATSGLEMAQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEG
EQTNYVQGANIAGFVKVADAMLAQGVI