Protein Info for b1702 in Escherichia coli BW25113

Name: pps
Annotation: phosphoenolpyruvate synthase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01418: phosphoenolpyruvate synthase
  • amino acids 9 to 792 (784 residues), 1290.5 bits, E=0
• PF01326: PPDK_N
  • amino acids 22 to 349 (328 residues), 434.1 bits, E=5.3e-134
• PF00391: PEP-utilizers
  • amino acids 386 to 457 (72 residues), 100.1 bits, E=6.5e-33
• PF02896: PEP-utilizers_C
  • amino acids 478 to 789 (312 residues), 201 bits, E=3.6e-63

Best Hits

Swiss-Prot: 100% identical to PPSA_ECOLI: Phosphoenolpyruvate synthase (ppsA) from Escherichia coli (strain K12)

KEGG orthology group: K01007, pyruvate, water dikinase [EC: 2.7.9.2] (inferred from 100% identity to ecg:E2348C_1787)

MetaCyc: 100% identical to phosphoenolpyruvate synthetase (Escherichia coli K-12 substr. MG1655)
Pyruvate, water dikinase. [EC: 2.7.9.2]

Predicted SEED Role

"Phosphoenolpyruvate synthase (EC 2.7.9.2)" in subsystem Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 2.7.9.2)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (25/26 steps found)
• superpathway of N-acetylneuraminate degradation (22/22 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (19/19 steps found)
• superpathway of hexitol degradation (bacteria) (18/18 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (17/17 steps found)
• gluconeogenesis I (13/13 steps found)
• glycolysis I (from glucose 6-phosphate) (13/13 steps found)
• superpathway of anaerobic sucrose degradation (17/19 steps found)
• glycolysis II (from fructose 6-phosphate) (11/11 steps found)
• glycolysis V (Pyrococcus) (7/10 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (9/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (22/56 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.9.2

Sequence Analysis Tools

See P23538 at UniProt or InterPro

Protein Sequence (792 amino acids)

>b1702 phosphoenolpyruvate synthase (NCBI) (Escherichia coli BW25113)
MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVSVPNGFATTADAFNQFL
DQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQPELENAIREAYAQLSADDE
NASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAVKHVFASLFNDRAISYRVHQG
YDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQVVFITSAWGLGEMVVQGAVNPDE
FYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHGKQVKIEDVPQEQRDIFSLTNEEVQ
ELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQARPETVRSRGQVMERYTLHSQGKIIA
EGRAIGHRIGAGPVKVIHDISEMNRIEPGDVLVTDMTDPDWEPIMKKASAIVTNRGGRTC
HAAIIARELGIPAVVGCGDATERMKDGENVTVSCAEGDTGYVYAELLEFSVKSSSVETMP
DLPLKVMMNVGNPDRAFDFACLPNEGVGLARLEFIINRMIGVHPRALLEFDDQEPQLQNE
IREMMKGFDSPREFYVGRLTEGIATLGAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDE
ENPMLGFRGAGRYVSDSFRDCFALECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEE
LARQGLKRGENGLKIIMMCEIPSNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVS
ELFDERNDAVKALLSMAIRAAKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTV
VQTWLSLAELKK