Protein Info for b1651 in Escherichia coli BW25113

Name: gloA
Annotation: glyoxalase I, Ni-dependent (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 135 TIGR00068: lactoylglutathione lyase" amino acids 2 to 134 (133 residues), 229.9 bits, see alignment E=4.2e-73 PF00903: Glyoxalase" amino acids 2 to 123 (122 residues), 99.9 bits, see alignment E=2.1e-32 PF13669: Glyoxalase_4" amino acids 5 to 109 (105 residues), 30.6 bits, see alignment E=5.7e-11 PF18029: Glyoxalase_6" amino acids 7 to 123 (117 residues), 24.5 bits, see alignment E=5.5e-09

Best Hits

Swiss-Prot: 100% identical to LGUL_ECOLI: Lactoylglutathione lyase (gloA) from Escherichia coli (strain K12)

KEGG orthology group: K01759, lactoylglutathione lyase [EC: 4.4.1.5] (inferred from 100% identity to eco:b1651)

MetaCyc: 100% identical to lactoylglutathione lyase (Escherichia coli K-12 substr. MG1655)
Lactoylglutathione lyase. [EC: 4.4.1.5]

Predicted SEED Role

"Lactoylglutathione lyase (EC 4.4.1.5)" in subsystem Glutathione: Non-redox reactions or Methylglyoxal Metabolism (EC 4.4.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.4.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P0AC81 at UniProt or InterPro

Protein Sequence (135 amino acids)

>b1651 glyoxalase I, Ni-dependent (NCBI) (Escherichia coli BW25113)
MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYN
WGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGPVKGGTTVIAFVEDPDGYK
IELIEEKDAGRGLGN