Protein Info for b1542 in Escherichia coli BW25113

Name: ydfI
Annotation: predicted mannonate dehydrogenase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to YDFI_ECOLI: Uncharacterized oxidoreductase YdfI (ydfI) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to eco:b1542)

MetaCyc: 58% identical to D-mannonate oxidoreductase (Escherichia coli K-12 substr. MG1655)
Fructuronate reductase. [EC: 1.1.1.57]

Predicted SEED Role

"D-mannonate oxidoreductase (EC 1.1.1.57)" in subsystem D-Galacturonate and D-Glucuronate Utilization (EC 1.1.1.57)

MetaCyc Pathways

superpathway of hexuronide and hexuronate degradation (10/10 steps found)
superpathway of β-D-glucuronosides degradation (7/7 steps found)
D-fructuronate degradation (4/4 steps found)
superpathway of microbial D-galacturonate and D-glucuronate degradation (19/31 steps found)

KEGG Metabolic Maps

Pentose and glucuronate interconversions

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.57

Use Curated BLAST to search for 1.1.1.57

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P77260 at UniProt or InterPro

Protein Sequence (486 amino acids)

>b1542 predicted mannonate dehydrogenase (NCBI) (Escherichia coli BW25113)
MGNNLLSAKATLPVYDLNNLAPRIVHLGFGAFHRAHQGVYADILATEHFSDWGYYEVNLI
GGEQQIADLQQQDNLYTVAEMSADVWTARVVGVVKKALHVQIDGLETVLAAMCEPQIAIV
SLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATGVIVEALARRKAAGLPAFTVM
SCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFPSTMVDRIVPAVTEDTLAKIEQL
TGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDVLPYEEMKLRMLNGSHSFLA
YLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAPTLKVQGVDLQDYANRLIARYSNPALR
HRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKFDLLALGVAGWMRYVGGVDEQGNPIEISD
PLLPVIQKAVQSSAEGKARVQSLLAIKAIFGDDLPDNSLFTARVTETYLSLLAHGAKATV
AKYSVK