Protein Info for b1525 in Escherichia coli BW25113

Name: b1525
Annotation: putative aldehyde dehydrogenase (VIMSS)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 462 PF00171: Aldedh" amino acids 10 to 457 (448 residues), 470.5 bits, see alignment E=2.5e-145

Best Hits

Swiss-Prot: 100% identical to SAD_ECOLI: Succinate semialdehyde dehydrogenase [NAD(P)+] Sad (sad) from Escherichia coli (strain K12)

KEGG orthology group: K08324, aldehyde dehydrogenase family protein [EC: 1.2.1.-] (inferred from 100% identity to eco:b1525)

MetaCyc: 100% identical to succinate semialdehyde dehydrogenase (NAD(P)+) Sad (Escherichia coli K-12 substr. MG1655)
Succinate-semialdehyde dehydrogenase. [EC: 1.2.1.24]; Glutarate-semialdehyde dehydrogenase. [EC: 1.2.1.24, 1.2.1.20]

Predicted SEED Role

"Succinate-semialdehyde dehydrogenase [NAD] (EC 1.2.1.24); Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16)" (EC 1.2.1.16, EC 1.2.1.24)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.-, 1.2.1.16

Use Curated BLAST to search for 1.2.1.- or 1.2.1.16 or 1.2.1.20 or 1.2.1.24

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P76149 at UniProt or InterPro

Protein Sequence (462 amino acids)

>b1525 putative aldehyde dehydrogenase (VIMSS) (Escherichia coli BW25113)
MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRDWRETNIDYRAEKLRDI
GKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAEHGPAMLKAEPTLVENQQA
VIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAG
IPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGAAIGAQAGAALKKCVLELGGSDP
FIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVAAAAALKMGDP
RDEENALGPMARFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMT
AFREEMFGPVAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFIN
GYCASDARVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI