Protein Info for b1053 in Escherichia coli BW25113

Name: mdtG
Annotation: predicted drug efflux system (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 408 transmembrane" amino acids 16 to 40 (25 residues), see Phobius details amino acids 53 to 74 (22 residues), see Phobius details amino acids 86 to 104 (19 residues), see Phobius details amino acids 111 to 131 (21 residues), see Phobius details amino acids 142 to 162 (21 residues), see Phobius details amino acids 173 to 193 (21 residues), see Phobius details amino acids 220 to 245 (26 residues), see Phobius details amino acids 257 to 278 (22 residues), see Phobius details amino acids 290 to 308 (19 residues), see Phobius details amino acids 374 to 395 (22 residues), see Phobius details PF07690: MFS_1" amino acids 20 to 251 (232 residues), 120.8 bits, see alignment E=9.5e-39 amino acids 224 to 395 (172 residues), 81 bits, see alignment E=1.3e-26 PF12832: MFS_1_like" amino acids 24 to 381 (358 residues), 50.2 bits, see alignment E=3.2e-17 PF00083: Sugar_tr" amino acids 49 to 124 (76 residues), 28.4 bits, see alignment E=1.2e-10

Best Hits

Swiss-Prot: 100% identical to MDTG_ECOBW: Multidrug resistance protein MdtG (mdtG) from Escherichia coli (strain K12 / MC4100 / BW2952)

KEGG orthology group: K08161, MFS transporter, DHA1 family, multidrug resistance protein (inferred from 100% identity to eco:b1053)

MetaCyc: 100% identical to efflux pump MdtG (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-342; TRANS-RXN0-589

Predicted SEED Role

"Multidrug-efflux transporter, major facilitator superfamily (MFS) (TC 2.A.1)" in subsystem Multidrug Resistance Efflux Pumps

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P25744 at UniProt or InterPro

Protein Sequence (408 amino acids)

>b1053 predicted drug efflux system (NCBI) (Escherichia coli BW25113)
MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQLGVTGHSALNMWSGIVF
SITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMGLAQNIWQFLILRALLGLL
GGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPMAGGLLADSYGLRPVFFITAS
VLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNPKLVLSLFVTTLIIQVATGSIAP
ILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAPRLGKLGDRIGPEKILITALIFSVL
LLIPMSYVQTPLQLGILRFLLGAADGALLPAVQTLLVYNSSNQIAGRIFSYNQSFRDIGN
VTGPLMGAAISANYGFRAVFLVTAGVVLFNAVYSWNSLRRRRIPQVSN