Protein Info for b0910 in Escherichia coli BW25113

Name: cmk
Annotation: cytidylate kinase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00017: cytidylate kinase
  • amino acids 6 to 219 (214 residues), 323.5 bits, E=3.4e-101
• PF13189: Cytidylate_kin2
  • amino acids 7 to 186 (180 residues), 28.6 bits, E=1.5e-10
• PF02224: Cytidylate_kin
  • amino acids 8 to 221 (214 residues), 292.2 bits, E=2.4e-91

Best Hits

Swiss-Prot: 100% identical to KCY_SHIB3: Cytidylate kinase (cmk) from Shigella boydii serotype 18 (strain CDC 3083-94 / BS512)

KEGG orthology group: K00945, cytidylate kinase [EC: 2.7.4.14] (inferred from 100% identity to eco:b0910)

MetaCyc: 100% identical to cytidylate kinase (Escherichia coli K-12 substr. MG1655)
(Deoxy)nucleoside-phosphate kinase. [EC: 2.7.4.13, 2.7.4.14, 2.7.4.25]; 2.7.4.14,2.7.4.25 [EC: 2.7.4.13, 2.7.4.14, 2.7.4.25]

Predicted SEED Role

"Cytidylate kinase (EC 2.7.4.25)" (EC 2.7.4.25)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• superpathway of pyrimidine ribonucleosides salvage (10/10 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis II (7/7 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• superpathway of pyrimidine deoxyribonucleoside salvage (8/9 steps found)
• pyrimidine deoxyribonucleotide phosphorylation (4/4 steps found)
• superpathway of pyrimidine nucleobases salvage (4/4 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• UTP and CTP de novo biosynthesis (3/3 steps found)
• CMP phosphorylation (2/2 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• purine deoxyribonucleosides salvage (4/6 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.4.13 or 2.7.4.14 or 2.7.4.25

Sequence Analysis Tools

See P0A6I0 at UniProt or InterPro

Protein Sequence (227 amino acids)

>b0910 cytidylate kinase (NCBI) (Escherichia coli BW25113)
MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLALAALHHHVDVASEDAL
VPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQVAAFPRVREALLRRQRAF
RELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRMLQLQEKGFSVNFERLLAEIKE
RDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQYARQKLALA