Protein Info for b0871 in Escherichia coli BW25113

Name: poxB
Annotation: pyruvate dehydrogenase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02776: TPP_enzyme_N
  • amino acids 4 to 116 (113 residues), 108.2 bits, E=3.3e-35
• PF00205: TPP_enzyme_M
  • amino acids 192 to 319 (128 residues), 116.9 bits, E=8.4e-38
• PF02775: TPP_enzyme_C
  • amino acids 379 to 525 (147 residues), 146.4 bits, E=8.8e-47

Best Hits

Swiss-Prot: 100% identical to POXB_ECOLI: Pyruvate dehydrogenase [ubiquinone] (poxB) from Escherichia coli (strain K12)

KEGG orthology group: K00156, pyruvate dehydrogenase (quinone) [EC: 1.2.5.1] (inferred from 100% identity to eco:b0871)

MetaCyc: 100% identical to pyruvate oxidase (Escherichia coli K-12 substr. MG1655)
RXN-11496 [EC: 1.2.5.1]; RXN0-2022 [EC: 1.2.5.1]

Predicted SEED Role

"Pyruvate oxidase [ubiquinone, cytochrome] (EC 1.2.2.2)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 1.2.2.2)

MetaCyc Pathways

• pyruvate to cytochrome bd oxidase electron transfer (2/2 steps found)
• pyruvate to cytochrome bo oxidase electron transfer (1/2 steps found)
• pyruvate fermentation to acetoin (1/3 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.2.2 or 1.2.5.1

Sequence Analysis Tools

See P07003 at UniProt or InterPro

Protein Sequence (572 amino acids)

>b0871 pyruvate dehydrogenase (NCBI) (Escherichia coli BW25113)
MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEWMSTRHEEVAAFAAGAE
AQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQEL
FRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLPGDVALKPAPEGATMHWYHAP
QPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKELVEFAGKIKAPIVHALRGKEHV
EYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQFPYRAFYPTDAKIIQIDINPASIGA
HSKVDMALVGDIKSTLRALLPLVEEKADRKFLDKALEDYRDARKGLDDLAKPSEKAIHPQ
YLAQQISHFAADDAIFTCDVGTPTVWAARYLKMNGKRRLLGSFNHGSMANAMPQALGAQA
TEPERQVVAMCGDGGFSMLMGDFLSVVQMKLPVKIVVFNNSVLGFVAMEMKAGGYLTDGT
ELHDTNFARIAEACGITGIRVEKASEVDEALQRAFSIDGPVLVDVVVAKEELAIPPQIKL
EQAKGFSLYMLRAIISGRGDEVIELAKTNWLR