Protein Info for b0772 in Escherichia coli BW25113

Name: ybhC
Annotation: predicted pectinesterase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01095: Pectinesterase
  • amino acids 195 to 318 (124 residues), 39.6 bits, E=1.4e-14

Best Hits

Swiss-Prot: 100% identical to YBHC_ECOLI: Putative acyl-CoA thioester hydrolase YbhC (ybhC) from Escherichia coli (strain K12)

KEGG orthology group: K01051, pectinesterase [EC: 3.1.1.11] (inferred from 100% identity to eco:b0772)

Predicted SEED Role

"Pectinesterase (EC 3.1.1.11)" in subsystem D-Galacturonate and D-Glucuronate Utilization (EC 3.1.1.11)

MetaCyc Pathways

• pectin degradation II (3/5 steps found)
• pectin degradation I (1/3 steps found)

KEGG Metabolic Maps

• Pentose and glucuronate interconversions
• Starch and sucrose metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.1.11

Sequence Analysis Tools

See P46130 at UniProt or InterPro

Protein Sequence (427 amino acids)

>b0772 predicted pectinesterase (NCBI) (Escherichia coli BW25113)
MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPILSAKEAQNFDAQHYFA
SLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVDAAIIKRTNKRQYIAVMPG
EYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPADWRHDVNPRGKYMPGKPAWY
MYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENTLGDSVDAGNHPAVALRTDGDQV
QINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVTNSYIEGDVDIVSGRGAVVFDNTEF
RVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFNAFGDGVAQLGRSLDVDANTNGQVVIR
DSAINEGFNTAKPWADAVISNRPFAGNTGSVDDNDEIQRNLNDTNYNRMWEYNNRGVGSK
VVAEAKK