Protein Info for b0674 in Escherichia coli BW25113

Name: asnB
Annotation: asparagine synthetase B (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01536: asparagine synthase (glutamine-hydrolyzing)
  • amino acids 2 to 371 (370 residues), 427.5 bits, E=4.6e-132
• PF13522: GATase_6
  • amino acids 33 to 161 (129 residues), 135 bits, E=3.2e-43
• PF13537: GATase_7
  • amino acids 48 to 166 (119 residues), 145.5 bits, E=1.5e-46
• PF12481: DUF3700
  • amino acids 111 to 193 (83 residues), 37.7 bits, E=3.2e-13
• PF00733: Asn_synthase
  • amino acids 211 to 366 (156 residues), 189.3 bits, E=3.2e-59
  • amino acids 364 to 511 (148 residues), 128.7 bits, E=8.9e-41

Best Hits

Swiss-Prot: 100% identical to ASNB_ECOLI: Asparagine synthetase B [glutamine-hydrolyzing] (asnB) from Escherichia coli (strain K12)

KEGG orthology group: K01953, asparagine synthase (glutamine-hydrolysing) [EC: 6.3.5.4] (inferred from 100% identity to eco:b0674)

MetaCyc: 100% identical to asparagine synthetase B (Escherichia coli K-12 substr. MG1655)
Asparagine synthase (glutamine-hydrolyzing). [EC: 6.3.5.4]; Aspartate--ammonia ligase. [EC: 6.3.5.4, 6.3.1.1]; Glutamate synthase (NADPH). [EC: 6.3.5.4, 6.3.1.1, 1.4.1.13, 1.4.1.14, 1.4.7.1, 2.6.1.85, 3.5.1.2, 3.5.1.38, 4.1.3.27, 4.3.2.10, 4.3.3.6, 6.3.4.2, 6.3.5.13, 6.3.5.2, 6.3.5.5, 6.3.5.6, 6.3.5.7]

Predicted SEED Role

"Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)" in subsystem Cyanophycin Metabolism or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 6.3.5.4)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of chorismate metabolism (54/59 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (26/26 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of aromatic amino acid biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of arginine and polyamine biosynthesis (16/17 steps found)
• superpathway of L-tryptophan biosynthesis (13/13 steps found)
• superpathway of purine nucleotide salvage (13/14 steps found)
• L-histidine biosynthesis (10/10 steps found)
• superpathway of pyrimidine ribonucleosides salvage (10/10 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (11/12 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis II (8/8 steps found)
• L-arginine biosynthesis II (acetyl cycle) (9/10 steps found)
• superpathway of tetrahydrofolate biosynthesis (9/10 steps found)
• L-tryptophan biosynthesis (6/6 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis I (6/6 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• allantoin degradation IV (anaerobic) (8/9 steps found)
• guanosine ribonucleotides de novo biosynthesis (4/4 steps found)
• superpathway of L-aspartate and L-asparagine biosynthesis (4/4 steps found)
• superpathway of pyrimidine nucleobases salvage (4/4 steps found)
• L-citrulline degradation (3/3 steps found)
• UTP and CTP de novo biosynthesis (3/3 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• UMP biosynthesis III (5/6 steps found)
• 4-aminobenzoate biosynthesis I (2/2 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• ammonia assimilation cycle I (2/2 steps found)
• superpathway of L-asparagine biosynthesis (2/2 steps found)
• L-asparagine biosynthesis I (1/1 steps found)
• L-asparagine biosynthesis II (1/1 steps found)
• L-asparagine degradation I (1/1 steps found)
• L-glutamate biosynthesis IV (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-glutamine degradation II (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• GABA shunt II (3/4 steps found)
• L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• L-asparagine degradation III (mammalian) (2/3 steps found)
• superpathway of ammonia assimilation (plants) (2/3 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• ammonia assimilation cycle II (1/2 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• nitrogen remobilization from senescing leaves (5/8 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)
• 4-hydroxy-2(1H)-quinolone biosynthesis (2/5 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (1/4 steps found)
• acridone alkaloid biosynthesis (1/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (1/4 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)
• superpathway of candicidin biosynthesis (4/11 steps found)
• superpathway of quinolone and alkylquinolone biosynthesis (2/10 steps found)
• chloramphenicol biosynthesis (1/9 steps found)
• guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Aminoacyl-tRNA biosynthesis
• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Cyanoamino acid metabolism
• D-Glutamine and D-glutamate metabolism
• Drug metabolism - other enzymes
• Folate biosynthesis
• Glutamate metabolism
• Nitrogen metabolism
• Phenylalanine, tyrosine and tryptophan biosynthesis
• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.4.1.13, 1.4.1.14, 2.6.1.85, 3.5.1.2, 4.1.3.27, 6.3.1.1, 6.3.4.2, 6.3.5.2, 6.3.5.5

Use Curated BLAST to search for 1.4.1.13 or 1.4.1.14 or 1.4.7.1 or 2.6.1.85 or 3.5.1.2 or 3.5.1.38 or 4.1.3.27 or 4.3.2.10 or 4.3.3.6 or 6.3.1.1 or 6.3.4.2 or 6.3.5.13 or 6.3.5.2 or 6.3.5.4 or 6.3.5.5 or 6.3.5.6 or 6.3.5.7

Sequence Analysis Tools

See P22106 at UniProt or InterPro

Protein Sequence (554 amino acids)

>b0674 asparagine synthetase B (NCBI) (Escherichia coli BW25113)
MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNAILAHERLSIVDVNAGA
QPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEVILALYQEKGPEFLDDLQG
MFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVASEMKALVPVCRTIKEFPAGSY
LWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALEDSVKSHLMSDVPYGVLLSGGLDS
SIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLPGSPDLKAAQEVANHLGTVHHEIHF
TVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMSRKIKAMGIKMVLSGEGSDEVFGGYLY
FHKAPNAKELHEETVRKLLALHMYDCARANKAMSAWGVEARVPFLDKKFLDVAMRINPQD
KMCGNGKMEKHILRECFEAYLPASVAWRQKEQFSDGVGYSWIDTLKEVAAQQVSDQQLET
ARFRFPYNTPTSKEAYLYREIFEELFPLPSAAECVPGGPSVACSSAKAIEWDEAFKKMDD
PSGRAVGVHQSAYK