Protein Info for b0475 in Escherichia coli BW25113

Name: hemH
Annotation: ferrochelatase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to HEMH_ECOLI: Ferrochelatase (hemH) from Escherichia coli (strain K12)

KEGG orthology group: K01772, ferrochelatase [EC: 4.99.1.1] (inferred from 100% identity to eco:b0475)

MetaCyc: 100% identical to ferrochelatase (Escherichia coli K-12 substr. MG1655)
PROTOHEMEFERROCHELAT-RXN [EC: 4.98.1.1]

Predicted SEED Role

"Ferrochelatase, protoheme ferro-lyase (EC 4.99.1.1)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis (EC 4.99.1.1)

MetaCyc Pathways

superpathway of heme b biosynthesis from glutamate (10/10 steps found)
superpathway of heme b biosynthesis from uroporphyrinogen-III (6/6 steps found)
superpathway of heme b biosynthesis from glycine (7/8 steps found)
heme b biosynthesis I (aerobic) (4/4 steps found)
heme b biosynthesis II (oxygen-independent) (4/4 steps found)
heme b biosynthesis V (aerobic) (4/4 steps found)

KEGG Metabolic Maps

Porphyrin and chlorophyll metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.98.1.1 or 4.99.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P23871 at UniProt or InterPro

Protein Sequence (320 amino acids)

>b0475 ferrochelatase (NCBI) (Escherichia coli BW25113)
MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLLWWPLLRGVILPLRSPR
VAKLYASVWMEGGSPLMVYSRQQQQALAQRLPEMPVALGMSYGSPSLESAVDELLAEHVD
HIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIRDYADNHDYINALANSVRASF
AKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTRELASALGMAPEKVMMTFQSRFGRE
PWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLETLEEIAEQNREVFLGAGGKKYEYIP
ALNATPEHIEMMANLVAAYR