Protein Info for b0403 in Escherichia coli BW25113

Name: malZ
Annotation: maltodextrin glucosidase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 604 PF02903: Alpha-amylase_N" amino acids 2 to 104 (103 residues), 31.1 bits, see alignment E=2.3e-11 PF00128: Alpha-amylase" amino acids 177 to 493 (317 residues), 299.6 bits, see alignment E=4e-93

Best Hits

Swiss-Prot: 100% identical to MALZ_ECOLI: Maltodextrin glucosidase (malZ) from Escherichia coli (strain K12)

KEGG orthology group: K01187, alpha-glucosidase [EC: 3.2.1.20] (inferred from 100% identity to eco:b0403)

MetaCyc: 100% identical to maltodextrin glucosidase (Escherichia coli K-12 substr. MG1655)
Alpha-glucosidase. [EC: 3.2.1.20]; 3.2.1.20 [EC: 3.2.1.20]; 3.2.1.20 [EC: 3.2.1.20]; 3.2.1.20 [EC: 3.2.1.20]; 3.2.1.20 [EC: 3.2.1.20]

Predicted SEED Role

"Maltodextrin glucosidase (EC 3.2.1.20)" in subsystem Maltose and Maltodextrin Utilization (EC 3.2.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P21517 at UniProt or InterPro

Protein Sequence (604 amino acids)

>b0403 maltodextrin glucosidase (NCBI) (Escherichia coli BW25113)
MLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDNEEMSVPMHKQRSQPQPG
VTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMPPARLEQFAVDVPDIGPQWA
ADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILRDWDEPVTAQAGGSTFYGGDLD
GISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYRHVDPQFGGDGALLRLRHNTQQLG
MRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPESPWRDWYSFSDDGTALDWLGYASLPK
LDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGWRLDVVHMLGEAGGARNNMQHVAGITEA
AKETQPEAYIVGEHFGDARQWLQADVEDAAMNYRGFTFPLWGFLANTDISYDPQQIDAQT
CMAWMDNYRAGLSHQQQLRMFNQLDSHDTARFKTLLGRDIARLPLAVVWLFTWPGVPCIY
YGDEVGLDGKNDPFCRKPFPWQVEKQDTALFALYQRMIALRKKSQALRHGGCQVLYAEDN
VVVFVRVLNQQRVLVAINRGEACEVVLPASPFLNAVQWQCKEGHGQLTDGILALPAISAT
VWMN