Protein Info for b0397 in Escherichia coli BW25113

Name: sbcC
Annotation: exonuclease, dsDNA, ATP-dependent (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1048 TIGR00618: exonuclease SbcC" amino acids 1 to 1040 (1040 residues), 1455.8 bits, see alignment E=0 PF13476: AAA_23" amino acids 5 to 206 (202 residues), 100.1 bits, see alignment E=4.3e-32 PF13555: AAA_29" amino acids 26 to 61 (36 residues), 28 bits, see alignment (E = 2.2e-10) PF13558: SbcC_Walker_B" amino acids 931 to 1002 (72 residues), 49.5 bits, see alignment 6.3e-17

Best Hits

Swiss-Prot: 100% identical to SBCC_ECOLI: Nuclease SbcCD subunit C (sbcC) from Escherichia coli (strain K12)

KEGG orthology group: K03546, exonuclease SbcC (inferred from 100% identity to eco:b0397)

MetaCyc: 100% identical to ATP-dependent structure-specific DNA nuclease - SbcC subunit (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Exonuclease SbcC" in subsystem DNA repair, bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P13458 at UniProt or InterPro

Protein Sequence (1048 amino acids)

>b0397 exonuclease, dsDNA, ATP-dependent (NCBI) (Escherichia coli BW25113)
MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKTTLLDAICLALYHETPR
LSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRARNQPDGNLQVPRVELARCA
DGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAAFLNAKPKERAELLEELTGTE
IYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQVQSLTASLQVLTDEEKQLITAQQ
QEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKAQPQLAALSLAQPARNLRPHWERIA
EHSAALAHIRQQIEEVNTRLQSTMALRASIRHHAAKQSAELQQQQQSLNTWLQEHDRFRQ
WNNEPAGWRAQFSQQTSDREHLRQWQQQLTHAEQKLNALAAITLTLTADEVATALAQHAE
QRPLRQHLVALHGQIVPQQKRLAQLQVAIQNVTQEQTQRNAALNEMRQRYKEKTQQLADV
KTICEQEARIKTLEAQRAQLQAGQPCPLCGSTSHPAVEAYQALEPGVNQSRLLALENEVK
KLGEEGATLRGQLDAITKQLQRDENEAQSLRQDEQALTQQWQAVTASLNITLQPLDDIQP
WLDAQDEHERQLRLLSQRHELQGQIAAHNQQIIQYQQQIEQRQQLLLTTLTGYALTLPQE
DEEESWLATRQQEAQSWQQRQNELTALQNRIQQLTPILETLPQSDELPHCEETVVLENWR
QVHEQCLALHSQQQTLQQQDVLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLT
QLEQLKQNLENQRRQAQTLVTQTAETLAQHQQHRPDDGLALTVTVEQIQQELAQTHQKLR
ENTTSQGEIRQQLKQDADNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQ
GLTLDNLVHLANQQLTRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVS
LALALALSDLVSHKTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAM
KERIPVQIKVKKINGLGYSKLESTFAVK