Protein Info for b0355 in Escherichia coli BW25113

Name: frmB
Annotation: predicted esterase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 277 TIGR02821: S-formylglutathione hydrolase" amino acids 2 to 274 (273 residues), 392.8 bits, see alignment E=4.6e-122 PF00756: Esterase" amino acids 21 to 268 (248 residues), 266.9 bits, see alignment E=1.1e-83

Best Hits

Swiss-Prot: 100% identical to SFGH1_ECODH: S-formylglutathione hydrolase FrmB (frmB) from Escherichia coli (strain K12 / DH10B)

KEGG orthology group: K01070, S-formylglutathione hydrolase [EC: 3.1.2.12] (inferred from 100% identity to eco:b0355)

MetaCyc: 100% identical to S-formylglutathione hydrolase FrmB (Escherichia coli K-12 substr. MG1655)
S-formylglutathione hydrolase. [EC: 3.1.2.12]

Predicted SEED Role

"S-formylglutathione hydrolase (EC 3.1.2.12)" in subsystem Glutathione-dependent pathway of formaldehyde detoxification (EC 3.1.2.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.2.12

Use Curated BLAST to search for 3.1.2.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P51025 at UniProt or InterPro

Protein Sequence (277 amino acids)

>b0355 predicted esterase (NCBI) (Escherichia coli BW25113)
MELIEKHVSFGGWQNMYRHYSQSLKCEMNVGVYLPPKAANEKLPVLYWLSGLTCNEQNFI
TKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGFYLNATQAPWNEHYKMYDY
IRNELPDLVMHHFPATAKKSISGHSMGGLGALVLALRNPDEYVSVSAFSPIVSPSQVPWG
QQAFAAYLAENKDAWLDYDPVSLISQGQRVAEIMVDQGLSDDFYAEQLRTPNLEKICQEM
NIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNMR