Protein Info for b0343 in Escherichia coli BW25113

Name: lacY
Annotation: galactoside permease (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 417 transmembrane" amino acids 10 to 34 (25 residues), see Phobius details amino acids 46 to 66 (21 residues), see Phobius details amino acids 75 to 95 (21 residues), see Phobius details amino acids 101 to 123 (23 residues), see Phobius details amino acids 143 to 162 (20 residues), see Phobius details amino acids 168 to 187 (20 residues), see Phobius details amino acids 222 to 239 (18 residues), see Phobius details amino acids 260 to 283 (24 residues), see Phobius details amino acids 291 to 310 (20 residues), see Phobius details amino acids 316 to 337 (22 residues), see Phobius details amino acids 349 to 369 (21 residues), see Phobius details amino acids 381 to 401 (21 residues), see Phobius details PF01306: LacY_symp" amino acids 2 to 412 (411 residues), 683.4 bits, see alignment E=1.6e-209 TIGR00882: oligosaccharide:H+ symporter" amino acids 6 to 401 (396 residues), 691.8 bits, see alignment E=1.4e-212 PF12832: MFS_1_like" amino acids 10 to 379 (370 residues), 77.3 bits, see alignment E=1.8e-25 PF07690: MFS_1" amino acids 14 to 333 (320 residues), 77.4 bits, see alignment E=1.6e-25

Best Hits

Swiss-Prot: 100% identical to LACY_ECOLI: Lactose permease (lacY) from Escherichia coli (strain K12)

KEGG orthology group: K02532, MFS transporter, OHS family, lactose permease (inferred from 100% identity to eco:b0343)

MetaCyc: 100% identical to lactose permease (Escherichia coli K-12 substr. MG1655)
RXN-17755; RXN0-7215; RXN0-7217; TRANS-RXN-24; TRANS-RXN-94

Predicted SEED Role

"Lactose permease" in subsystem Fructooligosaccharides(FOS) and Raffinose Utilization or Lactose and Galactose Uptake and Utilization or Lactose utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P02920 at UniProt or InterPro

Protein Sequence (417 amino acids)

>b0343 galactoside permease (NCBI) (Escherichia coli BW25113)
MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSDTGIIFAAISLFSLLFQ
PLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYNILVGSIVGGIYLGFCFNA
GAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGIMFTINNQFVFWLGSGCALIL
AVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFRQPKLWFLSLYVIGVSCTYDVFD
QQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIMFFAPLIINRIGGKNALLLAGTIMS
VRIIGSSFATSALEVVILKTLHMFEVPFLLVGCFKYITSQFEVRFSATIYLVCFCFFKQL
AMIFMSVLAGNMYESIGFQGAYLVLGLVALGFTLISVFTLSGPGPLSLLRRQVNEVA