Protein Info for b0311 in Escherichia coli BW25113

Name: betA
Annotation: choline dehydrogenase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 556 PF00732: GMC_oxred_N" amino acids 3 to 300 (298 residues), 214 bits, see alignment E=2.4e-67 TIGR01810: choline dehydrogenase" amino acids 4 to 537 (534 residues), 1035.8 bits, see alignment E=0 PF05199: GMC_oxred_C" amino acids 393 to 527 (135 residues), 145.9 bits, see alignment E=1.2e-46

Best Hits

Swiss-Prot: 100% identical to BETA_ECODH: Oxygen-dependent choline dehydrogenase (betA) from Escherichia coli (strain K12 / DH10B)

KEGG orthology group: K00108, choline dehydrogenase [EC: 1.1.99.1] (inferred from 100% identity to eco:b0311)

MetaCyc: 100% identical to choline dehydrogenase (Escherichia coli K-12 substr. MG1655)
Choline dehydrogenase. [EC: 1.1.99.1]; 1.1.99.1 [EC: 1.1.99.1]; RXN0-7231 [EC: 1.1.99.1]

Predicted SEED Role

"Choline dehydrogenase (EC 1.1.99.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.1.99.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P17444 at UniProt or InterPro

Protein Sequence (556 amino acids)

>b0311 choline dehydrogenase (NCBI) (Escherichia coli BW25113)
MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFRTQMPAALAFPLQGKRY
NWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNALDLDNWAQEPGLENWSYLD
CLPYYRKAETRDMGENDYHGGDGPVSVTTSKPGVNPLFEAMIEAGVQAGYPRTDDLNGYQ
QEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRTHAMTDHIIFDGKRAVGVEWLEG
DSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAELLAEFDIPLVHELPGVGENLQDHL
EMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGGTGVGASNHFEAGGFIRSREEFAWPNI
QYHFLPVAINYNGSNAVKEHGFQCHVGSMRSPSRGHVRIKSRDPHQHPAILFNYMSHEQD
WQEFRDAIRITREIMHQPALDQYRGREISPGVECQTDEQLDEFVRNHAETAFHPCGTCKM
GYDEMSVVDGEGRVHGLEGLRVVDASIMPQIITGNLNATTIMIGEKIADMIRGQEALPRS
TAGYFVANGMPVRAKK