Protein Info for b0284 in Escherichia coli BW25113
Name: yagR
Annotation: predicted oxidoreductase with molybdenum-binding domain (NCBI)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to PAOC_ECOLI: Aldehyde oxidoreductase molybdenum-binding subunit PaoC (paoC) from Escherichia coli (strain K12)
KEGG orthology group: K11177, xanthine dehydrogenase YagR molybdenum-binding subunit [EC: 1.17.1.4] (inferred from 100% identity to eco:b0284)MetaCyc: 100% identical to aldehyde dehydrogenase, molybdenum cofactor-binding subunit (Escherichia coli K-12 substr. MG1655)
Carboxylate reductase. [EC: 1.2.99.6]; 1.2.98.- [EC: 1.2.99.6]; 1.2.98.- [EC: 1.2.99.6]
Predicted SEED Role
"Periplasmic aromatic aldehyde oxidoreductase, molybdenum binding subunit YagR"
MetaCyc Pathways
- purine nucleotides degradation II (aerobic) (11/11 steps found)
- adenosine nucleotides degradation II (5/5 steps found)
- purine nucleotides degradation I (plants) (10/12 steps found)
- adenosine nucleotides degradation I (7/8 steps found)
- guanosine nucleotides degradation II (4/4 steps found)
- guanosine nucleotides degradation III (4/4 steps found)
- inosine 5'-phosphate degradation (4/4 steps found)
- superpathway of guanosine nucleotides degradation (plants) (5/6 steps found)
- guanosine nucleotides degradation I (3/4 steps found)
- superpathway of purines degradation in plants (13/18 steps found)
- ureide biosynthesis (5/7 steps found)
- purine nucleobases degradation II (anaerobic) (17/24 steps found)
- caffeine degradation III (bacteria, via demethylation) (1/7 steps found)
- theophylline degradation (1/9 steps found)
- caffeine degradation IV (bacteria, via demethylation and oxidation) (1/10 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.17.1.4
Use Curated BLAST to search for 1.17.1.4 or 1.2.99.6
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See P77489 at UniProt or InterPro
Protein Sequence (732 amino acids)
>b0284 predicted oxidoreductase with molybdenum-binding domain (NCBI) (Escherichia coli BW25113) MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEEAPNAAYGYIVGSAIAK GRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGGPTIEHYHQAIALVVAETF EQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEDTPDKNVGDFDGAFTSAAVKIDATY TTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRTDLAKTLKVPVENVRIISPYIGG GFGGKLFLRSDALLAALAARAVKRPVKVMLPRPSIPNNTTHRPATLQHLRIGADQSGKIT AISHESWSGNLPGGTPETAVQQSELLYAGANRHTGLRLATLDLPEGNAMRAPGEAPGLMA LEIAIDELAEKAGIDPVEFRILNDTQVDPADPTRCFSRRQLIECLRTGADKFGWKQRNAT PGQVRDGEWLVGHGVAAGFRNNLLEKSGARVHLEQNGTVTVETDMTDIGTGSYTILAQTA AEMLGVPLEQVAVHLGDSSFPVSAGSGGQWGANTSTSGVYAACMKLREMIASAVGFDPEQ SQFADGKITNGTRSATLHEATAGGRLTAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATG EVRVRRMLAVCAAGRILNPKTARSQVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYE VPVHADIPKQEVIFLDDTDPISSPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPIT LDKLLDKLPDVV