Protein Info for b0237 in Escherichia coli BW25113

Name: pepD
Annotation: aminoacyl-histidine dipeptidase (peptidase D) (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01893: Xaa-His dipeptidase
  • amino acids 8 to 482 (475 residues), 615.2 bits, E=3.8e-189
• PF01546: Peptidase_M20
  • amino acids 73 to 480 (408 residues), 59.4 bits, E=4.8e-20
• PF07687: M20_dimer
  • amino acids 207 to 294 (88 residues), 46.3 bits, E=3.7e-16

Best Hits

Swiss-Prot: 100% identical to PEPD_ECOLI: Cytosol non-specific dipeptidase (pepD) from Escherichia coli (strain K12)

KEGG orthology group: K01270, aminoacylhistidine dipeptidase [EC: 3.4.13.3] (inferred from 100% identity to eco:b0237)

MetaCyc: 100% identical to peptidase D (Escherichia coli K-12 substr. MG1655)
Cytosol nonspecific dipeptidase. [EC: 3.4.13.18]; 3.4.13.18 [EC: 3.4.13.18]; 3.4.13.18 [EC: 3.4.13.18]

Predicted SEED Role

"Aminoacyl-histidine dipeptidase (Peptidase D) (EC 3.4.13.3)" (EC 3.4.13.3)

MetaCyc Pathways

• muropeptide degradation (4/4 steps found)
• γ-glutamyl cycle (5/6 steps found)
• glutathione degradation (DUG pathway) (2/2 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Arginine and proline metabolism
• Glutathione metabolism
• Histidine metabolism
• Urea cycle and metabolism of amino groups
• beta-Alanine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.13.18 or 3.4.13.3

Sequence Analysis Tools

See P15288 at UniProt or InterPro

Protein Sequence (485 amino acids)

>b0237 aminoacyl-histidine dipeptidase (peptidase D) (NCBI) (Escherichia coli BW25113)
MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKGFHVERDQVGNILIRKP
ATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDGEWVKARGTTLGADNGIGM
ASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQGNWLQADILINTDSEEEGEIYMGC
AGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGGEIHVGLGNANKLLVRFLAGHAE
ELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVLKSLVNTYQEILKNELAEKEKNLAL
LLDSVANDKAALIAKSRDTFIRLLNATPNGVIRNSDVAKGVVETSLNVGVVTMTDNNVEI
HCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEAKGAYPGWQPDANSPVMHLVRETYQRLFN
KTPNIQIIHAGLECGLFKKPYPEMDMVSIGPTITGPHSPDEQVHIESVGHYWTLLTELLK
EIPAK