Protein Info for b0225 in Escherichia coli BW25113

Name: yafQ
Annotation: predicted toxin of the YafQ-DinJ toxin-antitoxin system (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 25 50 75 92 transmembrane" amino acids 31 to 47 (17 residues), see Phobius details TIGR00053: addiction module toxin component, YafQ family" amino acids 4 to 91 (88 residues), 120.1 bits, see alignment E=5e-39 PF15738: YafQ_toxin" amino acids 5 to 92 (88 residues), 109 bits, see alignment E=6.1e-36 TIGR02385: addiction module toxin, RelE/StbE family" amino acids 6 to 91 (86 residues), 89.1 bits, see alignment E=2.1e-29

Best Hits

Swiss-Prot: 100% identical to YAFQ_ECOLI: mRNA interferase toxin YafQ (yafQ) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to eco:b0225)

MetaCyc: 100% identical to ribosome-dependent mRNA interferase toxin YafQ (Escherichia coli K-12 substr. MG1655)
3.1.26.-

Predicted SEED Role

"YafQ toxin protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q47149 at UniProt or InterPro

Protein Sequence (92 amino acids)

>b0225 predicted toxin of the YafQ-DinJ toxin-antitoxin system (NCBI) (Escherichia coli BW25113)
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYR
DAHVEPDWILIYKLTDKLLRFERTGTHAALFG