Protein Info for b0219 in Escherichia coli BW25113
Name: yafV
Annotation: predicted C-N hydrolase family amidase, NAD(P)-binding (NCBI)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to YAFV_ECOLI: Omega-amidase YafV (yafV) from Escherichia coli (strain K12)
KEGG orthology group: K08590, carbon-nitrogen hydrolase family protein (inferred from 100% identity to eco:b0219)MetaCyc: 100% identical to 2-oxoglutaramate amidase (Escherichia coli K-12 substr. MG1655)
Omega-amidase. [EC: 3.5.1.111, 3.5.1.3]
Predicted SEED Role
"Aliphatic amidase AmiE (EC 3.5.1.4)" (EC 3.5.1.4)
MetaCyc Pathways
- acrylonitrile degradation I (1/2 steps found)
- indole-3-acetate biosynthesis III (bacteria) (1/2 steps found)
- indole-3-acetate biosynthesis IV (bacteria) (1/2 steps found)
- L-arginine degradation X (arginine monooxygenase pathway) (1/3 steps found)
- superpathway of acrylonitrile degradation (1/3 steps found)
- L-methionine salvage cycle III (5/11 steps found)
- L-methionine salvage cycle I (bacteria and plants) (5/12 steps found)
- S-methyl-5-thio-α-D-ribose 1-phosphate degradation I (1/7 steps found)
- L-methionine salvage cycle II (plants) (3/11 steps found)
- indole-3-acetate biosynthesis II (3/12 steps found)
- nicotine degradation I (pyridine pathway) (6/17 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Arginine and proline metabolism
- Benzoate degradation via CoA ligation
- Biosynthesis of plant hormones
- Cyanoamino acid metabolism
- Glutamate metabolism
- Phenylalanine metabolism
- Styrene degradation
- Tryptophan metabolism
- Urea cycle and metabolism of amino groups
Isozymes
No predicted isozymesUse Curated BLAST to search for 3.5.1.111 or 3.5.1.3 or 3.5.1.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q47679 at UniProt or InterPro
Protein Sequence (256 amino acids)
>b0219 predicted C-N hydrolase family amidase, NAD(P)-binding (NCBI) (Escherichia coli BW25113) MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMFTSGFAMEAAASSLAQD DVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTVHFYDKRHLFRMADEHLHY KAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLALYVANWPAPRSLHWQALLTAR AIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIATADAHQATRIDAELSMAALREY REKFPAWQDADEFRLW