Protein Info for b0194 in Escherichia coli BW25113

Name: proS
Annotation: prolyl-tRNA synthetase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00409: proline--tRNA ligase
  • amino acids 1 to 569 (569 residues), 940.3 bits, E=2.5e-287
• PF00587: tRNA-synt_2b
  • amino acids 96 to 459 (364 residues), 127.7 bits, E=8.4e-41
• PF04073: tRNA_edit
  • amino acids 257 to 376 (120 residues), 93.8 bits, E=1.2e-30
• PF03129: HGTP_anticodon
  • amino acids 475 to 568 (94 residues), 78.6 bits, E=4.9e-26

Best Hits

Swiss-Prot: 100% identical to SYP_ECOLI: Proline--tRNA ligase (proS) from Escherichia coli (strain K12)

KEGG orthology group: K01881, prolyl-tRNA synthetase [EC: 6.1.1.15] (inferred from 100% identity to eco:b0194)

MetaCyc: 100% identical to proline--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Cysteine--tRNA ligase. [EC: 6.1.1.16]; Proline--tRNA ligase. [EC: 6.1.1.16, 6.1.1.15]; 6.1.1.15 [EC: 6.1.1.16, 6.1.1.15]; 3.1.1.M26 [EC: 6.1.1.16, 6.1.1.15, 3.1.1.M26]

Predicted SEED Role

"Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type" (EC 6.1.1.15)

MetaCyc Pathways

• tRNA charging (21/21 steps found)
• anguibactin biosynthesis (2/8 steps found)
• pyoluteorin biosynthesis (1/7 steps found)
• thiocoraline biosynthesis (1/7 steps found)
• ergotamine biosynthesis (4/12 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• clorobiocin biosynthesis (1/10 steps found)
• pyochelin biosynthesis (1/11 steps found)
• superpathway of ergotamine biosynthesis (5/18 steps found)
• pyrrolomycin biosynthesis (1/14 steps found)
• sulfazecin biosynthesis (1/16 steps found)
• phosalacine biosynthesis (2/25 steps found)
• phosphinothricin tripeptide biosynthesis (2/25 steps found)
• colibactin biosynthesis (7/38 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis
• Cysteine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.16

Use Curated BLAST to search for 3.1.1.M26 or 6.1.1.15 or 6.1.1.16

Sequence Analysis Tools

See P16659 at UniProt or InterPro

Protein Sequence (572 amino acids)

>b0194 prolyl-tRNA synthetase (NCBI) (Escherichia coli BW25113)
MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGVRVLKKVENIVREE
MNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGERPFVLGPTHEEVITDLIRN
ELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDAYSFHTSQESLQETYDAMYAA
YSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGEDDVVFSDTSDYAANIELAEAIA
PKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEKTVKTLLVKAVEGSSFPQVALLVRG
DHELNEVKAEKLPQVASPLTFATEEEIRAVVKAGPGSLGPVNMPIPVVIDRTVAAMSDFA
AGANIDGKHYFGINWDRDVATPEVADIRNVVAGDPSPDGQGRLLIKRGIEVGHIFQLGTK
YSEALKASVQGEDGRNQILTMGCYGIGVTRVVAAAIEQNYDERGIVWPDAIAPFQVAILP
MNMHKSFRVQELAEKLYSELRAQGIEVLLDDRKERPGVMFADMELIGIPHTIVLGDRNLD
NDDIEYKYRRNGEKQLIKTGDIVEYLVKQIKG