Protein Info for b0159 in Escherichia coli BW25113

Name: pfs
Annotation: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01704: MTA/SAH nucleosidase
  • amino acids 2 to 229 (228 residues), 455.1 bits, E=2.1e-141
• PF01048: PNP_UDP_1
  • amino acids 2 to 226 (225 residues), 189.9 bits, E=2.4e-60

Best Hits

Swiss-Prot: 100% identical to MTNN_ECOL6: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K01243, S-adenosylhomocysteine/5'-methylthioadenosine nucleosidase [EC: 3.2.2.9] (inferred from 100% identity to eco:b0159)

MetaCyc: 100% identical to adenosylhomocysteine nucleosidase (Escherichia coli ATCC 25922)
Methylthioadenosine nucleosidase. [EC: 3.2.2.16, 3.2.2.9]; 3.2.2.9 [EC: 3.2.2.16, 3.2.2.9]; 3.2.2.9 [EC: 3.2.2.16, 3.2.2.9]

Predicted SEED Role

No annotation

MetaCyc Pathways

• S-adenosyl-L-methionine salvage I (4/4 steps found)
• autoinducer AI-2 biosynthesis II (Vibrio) (5/6 steps found)
• autoinducer AI-2 biosynthesis I (4/5 steps found)
• S-methyl-5'-thioadenosine degradation IV (1/1 steps found)
• S-methyl-5'-thioadenosine degradation I (1/2 steps found)
• L-cysteine biosynthesis VI (reverse transsulfuration) (4/7 steps found)
• 5'-deoxyadenosine degradation II (1/4 steps found)
• L-methionine salvage cycle I (bacteria and plants) (5/12 steps found)
• L-methionine salvage cycle II (plants) (3/11 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.2.16 or 3.2.2.9

Sequence Analysis Tools

See P0AF12 at UniProt or InterPro

Protein Sequence (232 amino acids)

>b0159 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (NCBI) (Escherichia coli BW25113)
MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVALLKSGIGKVAAALGAT
LLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADVTAFGYEYGQLPGCPAGFK
ADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAKIRHNFPQAIAVEMEATAIAH
VCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQSSLMVESLVQKLAHG