Protein Info for b0035 in Escherichia coli BW25113

Name: caiE
Annotation: possible synthesis of cofactor for carnitine racemase and dehydratase (VIMSS)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 196 transmembrane" amino acids 20 to 41 (22 residues), see Phobius details PF00132: Hexapep" amino acids 88 to 122 (35 residues), 32.2 bits, see alignment 3.1e-12

Best Hits

Swiss-Prot: 100% identical to CAIE_ECOBW: Carnitine operon protein CaiE (caiE) from Escherichia coli (strain K12 / MC4100 / BW2952)

KEGG orthology group: K08279, carnitine operon protein CaiE (inferred from 100% identity to eco:b0035)

MetaCyc: 100% identical to putative transferase CaiE (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Carnitine operon protein CaiE"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P39206 at UniProt or InterPro

Protein Sequence (196 amino acids)

>b0035 possible synthesis of cofactor for carnitine racemase and dehydratase (VIMSS) (Escherichia coli BW25113)
MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLRGDYGRLIVQAGANIQD
GCIMHGYCDTDTIVGENGHIGHGAILHGCLIGRDALVGMNSVIMDGAVIGEESIVAAMSF
VKAGFRGEKRQLLMGTPARAVRNVSDDELHWKRLNTKEYQDLVGRCHVSLHETQPLRQME
ENRPRLQGTTDVTPKR