Protein Info for b0026 in Escherichia coli BW25113

Name: ileS
Annotation: isoleucyl-tRNA synthetase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 938 TIGR00392: isoleucine--tRNA ligase" amino acids 15 to 843 (829 residues), 1031.1 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 28 to 640 (613 residues), 539.1 bits, see alignment E=2.4e-165 PF09334: tRNA-synt_1g" amino acids 58 to 120 (63 residues), 40.1 bits, see alignment 7.2e-14 PF13603: tRNA-synt_1_2" amino acids 223 to 358 (136 residues), 25.9 bits, see alignment E=2.2e-09 PF01406: tRNA-synt_1e" amino acids 574 to 648 (75 residues), 27.9 bits, see alignment E=5.5e-10 PF08264: Anticodon_1" amino acids 685 to 840 (156 residues), 101.4 bits, see alignment E=1.6e-32 PF06827: zf-FPG_IleRS" amino acids 898 to 927 (30 residues), 42.3 bits, see alignment (E = 1.8e-14)

Best Hits

Swiss-Prot: 96% identical to SYI_CITK8: Isoleucine--tRNA ligase (ileS) from Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)

KEGG orthology group: K01870, isoleucyl-tRNA synthetase [EC: 6.1.1.5] (inferred from 73% identity to aha:AHA_0682)

MetaCyc: 100% identical to isoleucine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Isoleucine--tRNA ligase. [EC: 6.1.1.5]; 6.1.1.5 [EC: 6.1.1.5]; 3.1.1.- [EC: 6.1.1.5]; RXN-23924 [EC: 6.1.1.5]

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P00956 at UniProt or InterPro

Protein Sequence (938 amino acids)

>b0026 isoleucyl-tRNA synthetase (NCBI) (Escherichia coli BW25113)
MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAAKKGKKTFILHDGPPYA
NGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPIELKVEQEYGKPGEKFTAA
EFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFKTEANIIRALGKIIGNGHLHK
GAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVDQDALKAKFAVSNVNGPISLVIW
TTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKDLVESVMQRIGVTDYTILGTVKGAE
LELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHTAPGHGPDDYVIGQKYGLETANPVGPD
GTYLPGTYPTLDGVNVFKANDIVVALLQEKGALLHVEKMQHSYPCCWRHKTPIIFRATPQ
WFVSMDQKGLRAQSLKEIKGVQWIPDWGQARIESMVANRPDWCISRQRTWGVPMSLFVHK
DTEELHPRTLELMEEVAKRVEVDGIQAWWDLDAKEILGDEADQYVKVPDTLDVWFDSGST
HSSVVDVRPEFAGHAADMYLEGSDQHRGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQG
RKMSKSIGNTVSPQDVMNKLGADILRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTAR
FLLANLNGFDPAKDMVKPEEMVVLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCS
VEMGSFYLDIIKDRQYTAKADSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPG
EREKYVFTGEWYEGLFGLADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAV
TLYAEPELSAKLTALGDELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEK
CPRCWHYTQDVGKVAEHAEICGRCVSNVAGDGEKRKFA