Protein Info for b0025 in Escherichia coli BW25113

Name: ribF
Annotation: hypothetical protein (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 313 PF06574: FAD_syn" amino acids 13 to 168 (156 residues), 196.6 bits, see alignment E=3.5e-62 TIGR00083: riboflavin biosynthesis protein RibF" amino acids 19 to 307 (289 residues), 409.9 bits, see alignment E=3.2e-127 PF01467: CTP_transf_like" amino acids 20 to 171 (152 residues), 21.7 bits, see alignment E=3e-08 PF01687: Flavokinase" amino acids 185 to 307 (123 residues), 143.7 bits, see alignment E=5.1e-46

Best Hits

Swiss-Prot: 100% identical to RIBF_ECOLI: Bifunctional riboflavin kinase/FMN adenylyltransferase (ribF) from Escherichia coli (strain K12)

KEGG orthology group: K11753, riboflavin kinase / FMN adenylyltransferase [EC: 2.7.1.26 2.7.7.2] (inferred from 100% identity to eco:b0025)

MetaCyc: 100% identical to bifunctional riboflavin kinase / FMN adenylyltransferase (Escherichia coli K-12 substr. MG1655)
FAD synthetase. [EC: 2.7.7.2]; Riboflavin kinase. [EC: 2.7.7.2, 2.7.1.26]

Predicted SEED Role

"Riboflavin kinase (EC 2.7.1.26) / FMN adenylyltransferase (EC 2.7.7.2)" in subsystem Riboflavin, FMN and FAD metabolism (EC 2.7.1.26, EC 2.7.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.26 or 2.7.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P0AG40 at UniProt or InterPro

Protein Sequence (313 amino acids)

>b0025 hypothetical protein (NCBI) (Escherichia coli BW25113)
MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGRKRNLPVMVMLFEPQPL
ELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAALTAQNFISDLLVKHLRVKF
LAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEGGVRISSTAVRQALADDNLAL
AESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQVSPVKGVYAVEVLGLGEKPLPGV
ANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVLRKKIRNEQRFASLDELKAQIARDE
LTAREFFGLTKPA