Protein Info for UW163_RS24005 in Ralstonia solanacearum UW163

Annotation: GMC family oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 647 transmembrane" amino acids 7 to 26 (20 residues), see Phobius details PF13450: NAD_binding_8" amino acids 9 to 43 (35 residues), 22.4 bits, see alignment (E = 1.9e-08) PF00732: GMC_oxred_N" amino acids 279 to 399 (121 residues), 48.8 bits, see alignment E=1.2e-16 PF05199: GMC_oxred_C" amino acids 547 to 630 (84 residues), 78.6 bits, see alignment E=1.1e-25

Best Hits

KEGG orthology group: K00540, [EC: 1.-.-.-] (inferred from 97% identity to rso:RS01675)

Predicted SEED Role

"Glucose-methanol-choline (GMC) oxidoreductase:NAD binding site" in subsystem Respiratory dehydrogenases 1

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (647 amino acids)

>UW163_RS24005 GMC family oxidoreductase (Ralstonia solanacearum UW163)
MKSKPDFLIVGSGGGGGTIAWVLASAGYDVLILEQGRNLAQQLRNPVLPFDPAVHDEELF
RLRKPDPKRRLRGDYNTFQDLGTQDPARAFDGGWTGSVLGGGSVLWGTWSFRPLPIDFRL
KTYYERTGELGQLQKEGYNVADWPLAYQEMMPYFHVAETLLGVSGDRAGMNRSIRESAWY
QDLRTRFGGEAWFGTDAQWLPDAAFPLGAYPTTPVGRVVWDGMDHKGMHPFPTPTAIVQP
GSAGFGTQAAIANALNQHRDELDGALWNRPVGQLWSNQIRQACNMCGYCGEYLCWGRTSP
KWGTADTTLRELGQRDNAEIRCNAKTLEVLYDAASRRATGVAYLDLTDPDHPRRVEQLAE
TVIISCGAVQTARLLLLSGPPDGLGNSSGQLGHNAMFHLFGLGMRTVLGEAFQGRLHGEL
GPTGNTSTFDTYFVRDGDGRWIKGGHLTSAAKKNPLEDAVNAVNGSSKAGAFLDQLLVNN
RTFEIRLTGDDLPCADNRVSLDPTYVDEYGVPVARISRRSISDAAGNKFVGPNEQRLDAA
AQGAMKAVFELYAAKQGALTAAPKPTQAILSLVGDHQMGTCRMGEDPSTSVVNRYGRLHD
VDNVFVVDSSFMPTGLGLNPMVTVVANALRVGTHIVTRLRQGSMPGH