Protein Info for UW163_RS22840 in Ralstonia solanacearum UW163

Annotation: DUF3416 domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1229 PF11896: GlgE_dom_N_S" amino acids 555 to 740 (186 residues), 190.9 bits, see alignment E=3.8e-60 PF00128: Alpha-amylase" amino acids 764 to 877 (114 residues), 23.4 bits, see alignment E=6.2e-09 PF21702: GLGE_C" amino acids 1150 to 1211 (62 residues), 42.3 bits, see alignment 1.1e-14

Best Hits

KEGG orthology group: None (inferred from 90% identity to rsl:RPSI07_mp0203)

Predicted SEED Role

"Alpha-amylase (EC 3.2.1.1)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis (EC 3.2.1.1)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1229 amino acids)

>UW163_RS22840 DUF3416 domain-containing protein (Ralstonia solanacearum UW163)
MTAPKTFATSAPRTPPSATSATTRTRRAAGDALRPVGKPASAEGQTPTMQAPSRGHPAES
RWTQPDARRRATVFAPAICYLHPLQHGPLDQWGSLLDRIASLGFDHVLIPPPFAVGAGGN
VFVTRDHARLHAALGGGPADAALRRLADDCRQRGLGLLMDLVIDRVATDAPLRADHPDWF
ASRTEHGDALDPRLPPSHLEVASFRHDDARCADAIGAWWAHQLRAWTALGIDGFRCEAPA
RLPPARWRTLIAATREATAARPVRFLAWTPGLDGEAVDALAQAGFDATFSSLRWWDFRAG
WMVEEHARLAAIAPPIATVEVPFGTRYGQAFGDAMTRERAYRRMLDVADVLGAGWLMPFG
FERGAMLPMLSERGDPTDQQWIHAHAAFDLSDAVRDVNATLRAAHADGTVAPRAELRMLT
GPDARMSALLRADGPDLRADEAATLLAINPDLHVGVSAHADRFLPGVAGGFTRGVALDLL
TEPTGSHDDALRTEAHPLAEIDLPPGGVQVWRVFRSRPVRRRPGVAPVIAPGVQQAEPAR
GDADNARANSAVAAPRIAIEHVTPAVDDGRFAVKRLVGDDILVEADVLMDGHDELAVELL
WRADDDAWQRVPMVPLGNDRWRGAFRPERLGRHRYAVAAWRGAFGTFRAELEKKHTAGVD
VSVELEEGAQLVAHAAGHAPKNAPLGALHQLADRLQTTAQDAAHRLKLLLDPATAHVMAQ
ASHFRPFHVQSAPYPLEVERSGAAFANWYELFPRSASGDEGRHGTFRDVVGRLGTIRAMG
FDVLYFPPIHPIGRTNRKGRNNSLRAGPGEPGSPYAIGAAEGGHDAVHPQLGTLEDFRRL
RDAAREQGLELALDFAIQCSPDHPWLREHPGWFAWRSDGSVRYAENPPKKYEDIVNVDFY
AADAKPALWRALRDVVMFWADEGVRLFRVDNPHTKPLPFWEWMIGEVRARHPDAIFLSEA
FTRPKGMYRLAKVGFSQSYTYFTWRHTRQEFTDYLTELTRTPVREYFRPHFFVNTPDINP
VFLQTSGRPGFLIRAALAATLSGLWGVYNGFELCEGQALPGKEEYLDSEKYQLRAWDHDR
PGNIVAEIALLNRIRRANPALHSHLGLQFQPASGEGILYFSKSTPAPLPTHPAELAGLTE
GADAQPERFGDNTLLVAISLDPHHPQEADIELPLWQWGLPDHGALMAENLVDGTRFVWRG
KRQHLRLEPERPFVIWRVRPALPSEEQPS