Protein Info for UW163_RS22065 in Ralstonia solanacearum UW163

Annotation: amino acid permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 474 transmembrane" amino acids 29 to 49 (21 residues), see Phobius details amino acids 55 to 74 (20 residues), see Phobius details amino acids 108 to 130 (23 residues), see Phobius details amino acids 136 to 157 (22 residues), see Phobius details amino acids 165 to 188 (24 residues), see Phobius details amino acids 205 to 230 (26 residues), see Phobius details amino acids 251 to 272 (22 residues), see Phobius details amino acids 292 to 316 (25 residues), see Phobius details amino acids 343 to 363 (21 residues), see Phobius details amino acids 369 to 394 (26 residues), see Phobius details amino acids 415 to 433 (19 residues), see Phobius details amino acids 439 to 458 (20 residues), see Phobius details PF00324: AA_permease" amino acids 27 to 459 (433 residues), 398.9 bits, see alignment E=3.1e-123 PF13520: AA_permease_2" amino acids 30 to 441 (412 residues), 120.5 bits, see alignment E=9.1e-39

Best Hits

Swiss-Prot: 53% identical to YTNA_BACSU: Uncharacterized amino acid permease YtnA (ytnA) from Bacillus subtilis (strain 168)

KEGG orthology group: K03293, amino acid transporter, AAT family (inferred from 71% identity to mno:Mnod_4992)

MetaCyc: 49% identical to D-serine/alanine/glycine:H+symporter (Escherichia coli K-12 substr. MG1655)
RXN0-5130; RXN0-5201; RXN0-5202; RXN0-5203; TRANS-RXN-62A; TRANS-RXN-62B

Predicted SEED Role

"D-serine/D-alanine/glycine transporter" in subsystem Glycine and Serine Utilization or Pyruvate Alanine Serine Interconversions

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (474 amino acids)

>UW163_RS22065 amino acid permease (Ralstonia solanacearum UW163)
MSTIDNMLAEERRHEAQDLRRGLTNRHIQMIALGGAIGVGLFLGAGRAISIAGPGLLLSY
AIGGIAVFFIMRALGELLTYRPVAGSFATYAEEFVGPFAGFATGWSYWFMWVTVGMAEIT
AVAVYVHYWFPDIPQWIPALSTLVVLYFVNRIAVAVFGELEFWFALIKIVMLVAMIVIGG
AILLFGVTPLGATASVSNLWSHQGFMPFGVLGVALTLQMVMFAFQGVELIGVTAGEAQDP
ERVLPQATNSVVWRILIFYIGALLVMMSLVPWTELKPGVSPFVFLFERMGIPGAASVINF
VVITAAASSCNSGIFSTGRMLYTLAQFRQAPQAFARINANHVPSTAVTFSAILMGAGVVL
NYVVPDEAFIWITSISLVSALWTWSIIMVAHLGYRKAVAAGRAKAVGFRMPGAPLANWLV
VAFMLVVSGLLALDPETRVALYIAPIWFALLGIGYRLAASANPSVRTATTSATR