Protein Info for UW163_RS21760 in Ralstonia solanacearum UW163

Annotation: chemotaxis protein CheA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 724 PF01627: Hpt" amino acids 8 to 105 (98 residues), 65.8 bits, see alignment E=8.5e-22 PF09078: CheY-binding" amino acids 171 to 232 (62 residues), 80.7 bits, see alignment 1.7e-26 PF02895: H-kinase_dim" amino acids 306 to 368 (63 residues), 70.5 bits, see alignment E=3.2e-23 PF02518: HATPase_c" amino acids 416 to 552 (137 residues), 66 bits, see alignment E=9.9e-22 PF01584: CheW" amino acids 558 to 687 (130 residues), 100 bits, see alignment E=2.2e-32

Best Hits

KEGG orthology group: K03407, two-component system, chemotaxis family, sensor kinase CheA [EC: 2.7.13.3] (inferred from 94% identity to rsl:RPSI07_mp1397)

Predicted SEED Role

"Signal transduction histidine kinase CheA (EC 2.7.3.-)" in subsystem Bacterial Chemotaxis or Flagellar motility or Two-component regulatory systems in Campylobacter (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (724 amino acids)

>UW163_RS21760 chemotaxis protein CheA (Ralstonia solanacearum UW163)
MNLDLSQFFGAFFEEAEELLVDMERLLLDLDVANPSSDDLNAIFRCAHSIKGGAATFGFT
HMTELTHVLESILDRARTGTLQLRENMVDAFLETKDVLKSQLEAYRHEHPIDTATLEYMV
AKLNSLTAGEGAAAAPAAAAAPAAVAEPQAQAQAPASTEPPIGEIEGGLDIKLTDVSDED
CELIVTELKHLGTVLRHARSGRNSEIVLQSTCTPDDIIAVSCFIIDADQIVIAPHVAGQA
PAAAAPAAPVAEADATAAAVAAPVPAAAQTAVHAPAANEAARPAAAAAPVPSRPASAEKP
AVAAETSIRVGVEKVDQLINLVGELVITQAMLAQTASAFDPVLNERLFAGLSQLTRNARD
LQEAAMSIRMMPMDYVFNRFPRLVRDLAHKLGKQVELSTFGKSTELDKGLIERIIDPLTH
LVRNSLDHGIETPEARTAAGKEATGQLLLSAAHQGGNIVIEVSDDGQGLNRDKILKKARE
RGLPVSDNMTDDEINQLIFAPGFSTADQVTDVSGRGVGMDVVKQNIQSMGGYVEIQSQRG
KGTTIRIVLPLTLAILDGMSVKTGEEVFILPLSCVAESLQPRPEDIKAVPGGGRLLKVRN
EYLTLVPLYERFRITPSIADPSEGIVVILDSEGKKIALQIDELVGQQQVVVKNLETNYRK
VSGISGATILGDGSVALIIDVSALMRETRVGHSESAMRAVTAEKPETANAAPGGRFSTEA
MAAA