Protein Info for UW163_RS21715 in Ralstonia solanacearum UW163

Annotation: sugar phosphate isomerase/epimerase and 4-hydroxyphenylpyruvate domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01261: AP_endonuc_2
  • amino acids 25 to 267 (243 residues), 156.5 bits, E=1.6e-49
• PF14696: Glyoxalase_5
  • amino acids 288 to 418 (131 residues), 104.7 bits, E=7.1e-34
• PF00903: Glyoxalase
  • amino acids 436 to 549 (114 residues), 27.7 bits, E=4.8e-10

Best Hits

KEGG orthology group: K00457, 4-hydroxyphenylpyruvate dioxygenase [EC: 1.13.11.27] (inferred from 96% identity to rsl:RPSI07_mp1388)

Predicted SEED Role

"4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27)" in subsystem Aromatic amino acid degradation or Homogentisate pathway of aromatic compound degradation or Plastoquinone Biosynthesis or Tocopherol Biosynthesis (EC 1.13.11.27)

MetaCyc Pathways

• L-tyrosine degradation I (5/5 steps found)
• plastoquinol-9 biosynthesis I (1/3 steps found)
• L-phenylalanine degradation IV (mammalian, via side chain) (5/9 steps found)
• superpathway of plastoquinol biosynthesis (2/5 steps found)
• vitamin E biosynthesis (tocopherols) (1/7 steps found)

KEGG Metabolic Maps

• Phenylalanine metabolism
• Tyrosine metabolism
• Ubiquinone and menaquinone biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.13.11.27

Use Curated BLAST to search for 1.13.11.27

Protein Sequence (626 amino acids)

>UW163_RS21715 sugar phosphate isomerase/epimerase and 4-hydroxyphenylpyruvate domain-containing protein (Ralstonia solanacearum UW163)
MPSSPFPFRKSIATVSLSGTLPEKLEAAAAAGFDGVELFEHDLLTFDGTPADVRRIVRDL
GLEITLFQPFRDFEGMPDALRERALARAERKFDLMQELGTGLVLVCSNTQEHALPDPARA
AADLAEMAERAARRGLRVGFEALAWGRHVNRWRQAWDIVRQADHPALGLILDSFHTLSVG
DTLDGLEDVPADKLAFVQLADAPRLSMDALSWSRHYRNFPGQGELPVVEFTRKVIACGYR
GPLSLEIFNDEFRAAPARQTAVDGLRSLIWLESECGAGTLPVAPRFGGVEFLEFAVDYRT
GLALAAQLKALGFRHAGRHRSKAVDLYRQGGVNLILNSEQDSAAAEHFALHGPSVCALGL
RVDDAQRALARARALLCRDWQERIGANERRIPALRTPDGMLMYLVDGDGPRTIYDDDFIL
KPPPTASATDDEPPYRLDHIAQALPAHRMDTFVLFYRAVLGLQPLALQEIPDPYGLVRSR
AMVSADGKLRLPLSVSESGRTSTGRLVSAYAGAGIQHIALGTPRILETLADVVRGGARLL
HIPENYYDDLEARHPLSAEQVETLRDAAVLYDRDAHGEFLHAYTVPFHDRFFFELVQRDG
YAGFGAANAAVRMAAQAAMTPYRQEA