Protein Info for UW163_RS20970 in Ralstonia solanacearum UW163

Annotation: ketohydroxyglutarate aldolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01081: Aldolase
  • amino acids 3 to 199 (197 residues), 233 bits, E=1.1e-73
• TIGR01182: 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase
  • amino acids 4 to 205 (202 residues), 244.1 bits, E=4.2e-77

Best Hits

Swiss-Prot: 50% identical to ALKH_DICD3: KHG/KDPG aldolase (eda) from Dickeya dadantii (strain 3937)

KEGG orthology group: K01625, 2-dehydro-3-deoxyphosphogluconate aldolase / 4-hydroxy-2-oxoglutarate aldolase [EC: 4.1.2.14 4.1.3.16] (inferred from 93% identity to rsl:RPSI07_mp1220)

MetaCyc: 50% identical to 2-dehydro-3-deoxy-D-gluconate-6-phosphate aldolase (Dickeya dadantii 3937)
2-dehydro-3-deoxy-phosphogluconate aldolase. [EC: 4.1.2.14, 4.1.2.55]

Predicted SEED Role

"2-dehydro-3-deoxyphosphogluconate aldolase (EC 4.1.2.14) / 4-Hydroxy-2-oxoglutarate aldolase (EC 4.1.3.16)" in subsystem D-Galacturonate and D-Glucuronate Utilization or Entner-Doudoroff Pathway (EC 4.1.2.14, EC 4.1.3.16)

MetaCyc Pathways

• superpathway of glycolysis and the Entner-Doudoroff pathway (15/17 steps found)
• Entner-Doudoroff pathway I (9/9 steps found)
• Entner-Doudoroff shunt (2/2 steps found)
• Entner-Doudoroff pathway III (semi-phosphorylative) (6/9 steps found)
• trans-4-hydroxy-L-proline degradation I (3/5 steps found)
• D-galacturonate degradation I (3/5 steps found)
• D-fructuronate degradation (2/4 steps found)
• D-glucosaminate degradation (1/3 steps found)
• 4-deoxy-L-threo-hex-4-enopyranuronate degradation (2/5 steps found)
• 3,6-anhydro-α-L-galactopyranose degradation (3/7 steps found)
• superpathway of β-D-glucuronosides degradation (3/7 steps found)
• superpathway of hexuronide and hexuronate degradation (4/10 steps found)
• superpathway of microbial D-galacturonate and D-glucuronate degradation (13/31 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Glyoxylate and dicarboxylate metabolism
• Pentose and glucuronate interconversions
• Pentose phosphate pathway

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.2.14 or 4.1.2.55 or 4.1.3.16

Protein Sequence (208 amino acids)

>UW163_RS20970 ketohydroxyglutarate aldolase (Ralstonia solanacearum UW163)
MGIDSVVQAGPVIPVLQFQSVDEGVAVCRALHAGGIRTFEITLRTPVALEVIRAVARDLG
SDALVGAGTLTRPEHFQQARDAGAVFAVSPGITPALAQAQARSGLEWLPGIATPSELILA
QEFGLNTLKFFPAEAAGGIPMLKSIHGPFGDVRFCPTGGITPKTAPDYLALPNVVCVGGS
WMVPKDKIAAGDWAGITALAGEAAALAR